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Identification and Pathway Analysis of microRNAs with No Previous Involvement in Breast Cancer
- Source :
- PLoS ONE, Vol 7, Iss 3, p e31904 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- microRNA expression signatures can differentiate normal and breast cancer tissues and can define specific clinico-pathological phenotypes in breast tumors. In order to further evaluate the microRNA expression profile in breast cancer, we analyzed the expression of 667 microRNAs in 29 tumors and 21 adjacent normal tissues using TaqMan Low-density arrays. 130 miRNAs showed significant differential expression (adjusted P value = 0.05, Fold Change = 2) in breast tumors compared to the normal adjacent tissue. Importantly, the role of 43 of these microRNAs has not been previously reported in breast cancer, including several evolutionary conserved microRNA*, showing similar expression rates to that of their corresponding leading strand. The expression of 14 microRNAs was replicated in an independent set of 55 tumors. Bioinformatic analysis of mRNA targets of the altered miRNAs, identified oncogenes like ERBB2, YY1, several MAP kinases, and known tumor-suppressors like FOXA1 and SMAD4. Pathway analysis identified that some biological process which are important in breast carcinogenesis are affected by the altered microRNA expression, including signaling through MAP kinases and TP53 pathways, as well as biological processes like cell death and communication, focal adhesion and ERBB2-ERBB3 signaling. Our data identified the altered expression of several microRNAs whose aberrant expression might have an important impact on cancer-related cellular pathways and whose role in breast cancer has not been previously described.
- Subjects :
- Gene Expression
lcsh:Medicine
Bioinformatics
Biochemistry
Nucleic Acids
Molecular Cell Biology
Genes, Tumor Suppressor
Breast
RNA, Neoplasm
Extracellular Signal-Regulated MAP Kinases
lcsh:Science
skin and connective tissue diseases
Conserved Sequence
Regulation of gene expression
Multidisciplinary
Obstetrics and Gynecology
Genomics
MicroRNA Expression Profile
Middle Aged
Phenotype
Receptors, Estrogen
Oncology
Medicine
Female
Receptors, Progesterone
Research Article
Adult
Breast Neoplasms
Biology
Molecular Genetics
Breast cancer
Genome Analysis Tools
microRNA
Genetics
medicine
Humans
RNA, Messenger
Aged
Gene Expression Profiling
lcsh:R
Computational Biology
Correction
Oncogenes
medicine.disease
Fold change
Gene expression profiling
MicroRNAs
Cancer research
RNA
lcsh:Q
FOXA1
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1f4a36bf48461d546ff30526d7b4c777
- Full Text :
- https://doi.org/10.1371/journal.pone.0031904