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Protective effect of EC-18, a synthetic monoacetyldiglyceride on lung inflammation in a murine model induced by cigarette smoke and lipopolysaccharide
- Source :
- International immunopharmacology. 30
- Publication Year :
- 2014
-
Abstract
- The antler of Sika deer (Cervus nippon Temminck) has been used a natural medicine in Korea, China and Japan, and a monoacetyldiaglyceride (1-palmitoyl-2-linoleoyl-3-acetylglycerol, PLAG) was found in the antler of Sika deer as a constituent for immunomodulation. In this study, we investigated protective effects of EC-18 (a synthetic copy of PLAG) on inflammatory responses using a cigarette smoke with lipopolysaccharide (LPS)-induced airway inflammation model. Mice were exposed to cigarette smoke for 1h per day for 3days. Ten micrograms of LPS dissolved in 50μL of PBS was administered intra nasally 1h after the final cigarette smoke exposure. EC-18 was administered by oral gavage at doses of 30 and 60mg/kg for 3days. EC-18 significantly reduced the number of neutrophils, reactive oxygen species production, cytokines and elastase activity in bronchoalveolar lavage fluid (BALF) compared with the cigarette smoke and LPS induced mice. Histologically, EC-18 attenuated airway inflammation with a reduction in myeloperoxidase expression in lung tissue. Additionally, EC-18 inhibited the phosphorylation of NF-κB and IκB induced by cigarette smoke and LPS exposure. Our results show that EC-18 effectively suppresses neutrophilic inflammation induced by cigarette smoke and LPS exposure. In conclusion, this study suggests that EC-18 has therapeutic potential for the treatment of chronic obstructive pulmonary disease.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Male
Lipopolysaccharide
Neutrophils
Microgram
Immunology
Anti-Inflammatory Agents
Inflammation
Antlers
Pharmacology
Diglycerides
03 medical and health sciences
chemistry.chemical_compound
Mice
Pulmonary Disease, Chronic Obstructive
Cell Movement
medicine
Immunology and Allergy
Animals
Humans
chemistry.chemical_classification
Reactive oxygen species
Lung
biology
medicine.diagnostic_test
business.industry
Deer
Smoking
Pneumonia
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Bronchoalveolar lavage
chemistry
Myeloperoxidase
biology.protein
Phosphorylation
Cytokines
medicine.symptom
Inflammation Mediators
business
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 18781705
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- International immunopharmacology
- Accession number :
- edsair.doi.dedup.....1f2e161e0e9f2ee758b0043b660565ed