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An easier analysis of complex mixtures with highly resolved and sensitivity enhanced 2D quantitative NMR: application to tracking sugar phosphates in plants

Authors :
Michael Hodges
Françoise Gilard
Caroline Mauve
Jonathan Farjon
Nathalie Glab
Grégory Mouille
Soumaya Khlifi
Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO)
Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Plateforme Métabolisme-Métabolome [Orsay]
Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403))
Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Institut Jean-Pierre Bourgin (IJPB)
Institut National de la Recherche Agronomique (INRA)-AgroParisTech
CNRS
University of South-Paris-Saclay
University of Paris-Saclay
INRA of Versailles
LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]
French National Research Agency under an Investments for the Future program [ANR-11-IDEX-0003-02]
INRA
Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)
French National Research Agency under an Investments for the Future program INRA
ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010)
ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011)
Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Institut de Biologie des Plantes (IBP)
Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)
Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM)
Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST)
Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Source :
Analytical Methods, Analytical Methods, Royal Society of Chemistry, 2017, 9 (15), pp.2328--2333. ⟨10.1039/c7ay00316a⟩, Analytical Methods, Royal Society of Chemistry, 2017, 9 (15), pp.2328-2333. ⟨10.1039/C7AY00316A⟩, Analytical Methods, 2017, 9 (15), pp.2328-2333. ⟨10.1039/C7AY00316A⟩
Publication Year :
2017
Publisher :
HAL CCSD, 2017.

Abstract

International audience; The combination of standard techniques with high resolution and sensitivity enhanced advanced techniques allowed for characterizing and quantifying sugar phosphates in plants biomarkers of photorespiration and photosynthesis in plants.The analysis of complex mixtures by NMR is a challenge in (bio) chemistry due to signal overcrowding making the analysis difficult. Thus, new advanced quantitative 2D NMR techniques that lead to better resolved and more intense signals have been applied to extracts of plants for tracking biomarkers of the photosynthesis and photorespiration cycles.

Subjects

Subjects :
0106 biological sciences
0301 basic medicine
chemistry.chemical_classification
Sugar phosphates
Quantitative nmr
General Chemical Engineering
Plant composition
General Engineering
Analytical chemistry
Photosynthesis
Tracking (particle physics)
01 natural sciences
Analytical Chemistry
03 medical and health sciences
030104 developmental biology
chemistry
Photorespiration
[CHIM]Chemical Sciences
Sensitivity (control systems)
Biological system
Two-dimensional nuclear magnetic resonance spectroscopy
010606 plant biology & botany

Details

Language :
English
ISSN :
17599660
Database :
OpenAIRE
Journal :
Analytical Methods, Analytical Methods, Royal Society of Chemistry, 2017, 9 (15), pp.2328--2333. ⟨10.1039/c7ay00316a⟩, Analytical Methods, Royal Society of Chemistry, 2017, 9 (15), pp.2328-2333. ⟨10.1039/C7AY00316A⟩, Analytical Methods, 2017, 9 (15), pp.2328-2333. ⟨10.1039/C7AY00316A⟩
Accession number :
edsair.doi.dedup.....1f27cc7198c20e8a5c9f3d0e4990eab4
Full Text :
https://doi.org/10.1039/c7ay00316a⟩
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