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Integration of lyoplate based flow cytometry and computational analysis for standardized immunological biomarker discovery
- Source :
- PLoS ONE, Vol 8, Iss 7, p e65485 (2013), Villanova, F, Di Meglio, P, Inokuma, M, Aghaeepour, N, Perucha, E, Mollon, J, Nomura, L, Hernandez-fuentes, M, Cope, A, Prevost, A T, Heck, S, Maino, V, Lord, G, Brinkman, R R, Nestle, F O & Von Herrath, M G (ed.) 2013, ' Integration of Lyoplate Based Flow Cytometry and Computational Analysis for Standardized Immunological Biomarker Discovery ', PLoS ONE, vol. 8, no. 7, pp. e65485 . https://doi.org/10.1371/journal.pone.0065485, PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Discovery of novel immune biomarkers for monitoring of disease prognosis and response to therapy in immune-mediated inflammatory diseases is an important unmet clinical need. Here, we establish a novel framework for immunological biomarker discovery, comparing a conventional (liquid) flow cytometry platform (CFP) and a unique lyoplate-based flow cytometry platform (LFP) in combination with advanced computational data analysis. We demonstrate that LFP had higher sensitivity compared to CFP, with increased detection of cytokines (IFN-γ and IL-10) and activation markers (Foxp3 and CD25). Fluorescent intensity of cells stained with lyophilized antibodies was increased compared to cells stained with liquid antibodies. LFP, using a plate loader, allowed medium-throughput processing of samples with comparable intra- and inter-assay variability between platforms. Automated computational analysis identified novel immunophenotypes that were not detected with manual analysis. Our results establish a new flow cytometry platform for standardized and rapid immunological biomarker discovery with wide application to immune-mediated diseases.
- Subjects :
- Male
Immunofluorescence
lcsh:Medicine
Biochemistry
Molecular Cell Biology
Pathology
Computational analysis
Biomarker discovery
lcsh:Science
Disease prognosis
Immune System Proteins
Multidisciplinary
medicine.diagnostic_test
T Cells
FOXP3
Middle Aged
Flow Cytometry
Interleukin-10
Multidisciplinary Sciences
Medicine
Science & Technology - Other Topics
Female
Antibody
Research Article
Test Evaluation
Adult
General Science & Technology
Immune Cells
Immunology
Activation markers
Computational biology
Biology
Sensitivity and Specificity
Antibodies
Flow cytometry
Immunophenotyping
Interferon-gamma
Young Adult
Immune system
Diagnostic Medicine
MD Multidisciplinary
medicine
Humans
CELL
Inflammation
Science & Technology
IDENTIFICATION
lcsh:R
Proteins
Computational Biology
Reproducibility of Results
Immunologic Techniques
biology.protein
lcsh:Q
Cytometry
Biomarkers
General Pathology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1f25534310f42b0a71e5748d641512ad