Additional file 5: of Drosophila melanogaster retrotransposon and inverted repeat-derived endogenous siRNAs are differentially processed in distinct cellular locations

Work flow for high throughput sequencing and small RNA analysis (SMACR). (A) Drosophila cells were individually depleted of Dcr2, CPSF73, Symplekin, and GFP (or LacZ). An additional fifth sample was untreated. The untreated and GFP samples represent controls. RNA was isolated from each sample and fractionated into RNAs > than 200 nts and RNAs