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Horseradish peroxidase in plasma studied by gel filtration
- Source :
- Histochemie. Histochemistry. Histochimie. 28(1)
- Publication Year :
- 1971
-
Abstract
- The problem whether the molecular size of horseradish peroxidase is significantly altered when the enzyme is injected into the blood stream in tracer studies, has been studied by molecular sieve chromatography (gel filtration). The results obtained rule out the possibility that the horseradish peroxidase molecule (containing about 20% carbohydrates) is significantly degraded by carbohydrate-splitting enzymes or by proteases in the circulation into a smaller active unit. Furthermore, significant binding of peroxidase to plasma proteins or polymerization of the enzyme, has not been detected. It is concluded, therefore, that conclusions about the size of functional pores based on the known molecular weight (40000 daltons) are permitted, when the enzyme is used in permeability studies.
- Subjects :
- Proteases
Histology
Cell Membrane Permeability
Hydrolases
Size-exclusion chromatography
Molecular sieve
Horseradish peroxidase
Animals
Molecular Biology
chemistry.chemical_classification
Chromatography
biology
Chemistry
Cell Biology
Blood Proteins
Haplorhini
Plants
Blood proteins
Molecular Weight
Medical Laboratory Technology
Enzyme
Biochemistry
Polymerization
Peroxidases
Injections, Intravenous
biology.protein
Chromatography, Gel
Peroxidase
Peptide Hydrolases
Protein Binding
Subjects
Details
- ISSN :
- 00182222
- Volume :
- 28
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Histochemie. Histochemistry. Histochimie
- Accession number :
- edsair.doi.dedup.....1ef640882a41d94c1d34d4921e86492f