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MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Authors :
Michael V. Tran
James W. Ferguson
Lauren E. Cote
Daria Khuntsariya
Richard D. Fetter
Jennifer T. Wang
Stephen R. Wellard
Maria D. Sallee
Mariya Genova
Sani Eskinazi
Maria M. Magiera
Carsten Janke
Tim Stearns
Zdenek Lansky
Kang Shen
Jérémy Magescas
Jessica L. Feldman
Source :
bioRxiv
Publication Year :
2023
Publisher :
Cold Spring Harbor Laboratory, 2023.

Abstract

SummaryMicrotubule doublets (MTDs) are a well conserved compound microtubule structure found primarily in cilia. However, the mechanisms by which MTDs form and are maintainedin vivoremain poorly understood. Here, we characterize microtubule-associated protein 9 (MAP9) as a novel MTD-associated protein. We demonstrate thatC. elegansMAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. Loss of MAPH-9 caused ultrastructural MTD defects, dysregulated axonemal motor velocity, and perturbed cilia function. As we found that the mammalian ortholog MAP9 localized to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in supporting the structure of axonemal MTDs and regulating ciliary motors.

Subjects

Subjects :
Article

Details

Language :
English
Database :
OpenAIRE
Journal :
bioRxiv
Accession number :
edsair.doi.dedup.....1ee5bf1d6ac65979de522b3473fc41b7