Functional Properties and Structural Requirements of the Plasmid pMV158-encoded MobM relaxase domain

A crucial element in the horizontal transfer of mobilizable and conjugative plasmids is the relaxase, a single-stranded endonuclease that nicks the origin of transfer (oriT) of the plasmid DNA. The relaxase of the pMV158 mobilizable plasmid is MobM (494 residues). In solution, MobM forms a dimer through its C-terminal domain, which is proposed to anchor the protein to the cell membrane and to participate in type 4 secretion system (T4SS) protein-protein interactions. In order to gain a deeper insight into the structural MobM requirements for efficient DNA catalysis, we studied two endonuclease domain variants that include the first 199 or 243 amino acid residues (MobMN199 and MobMN243, respectively). Our results confirmed that the two proteins behaved as monomers in solution. Interestingly, MobMN243 relaxed supercoiled DNA and cleaved single-stranded oligonucleotides harboring oriTpMV158, whereas MobMN199 was active only on supercoiled DNA. Protein stability studies using gel electrophoresis and mass spectrometry showed increased susceptibility to degradation at the domain boundary between the N-and C-terminal domains, suggesting that the domains change their relative orientation upon DNA binding. Overall, these results demonstrate that MobMN243 is capable of nicking the DNA substrate independently of its topology and that the amino acids 200 to 243 modulate substrate specificity but not the nicking activity per se. These findings suggest that these amino acids are involved in positioning the DNA for the nuclease reaction rather than in the nicking mechanism itself. © 2013, American Society for Microbiology. [Journal]
This work was supported by the Spanish Ministry of Science and Innovation (grants BFU2008-02372/BMC, CONSOLIDER CSD 2006-23, and BFU2011-22588 to M.C. and grants CSD2008-00013, INTERMODS, and BFU2010-19597 to M.E.), the Generalitat de Catalunya (grant SGR2009-1309 to M.C.), the “La Caixa”/IRB Barcelona International PhD Programme Fellowship (IRB Barcelona call 01/09/FLC to R.P.), and the European Commission (FP7 Cooperation Project SILVER-GA no. 260644 to M.C.)