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Binding and photocleavage of cationic porphyrin–phenylpiperazine hybrids to DNA
- Source :
- Biophysical Chemistry. 119:295-302
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- The binding properties of cationic porphyrin–phenylpiperazine hybrids to calf thymus (CT) DNA were investigated by using absorption, fluorescence and circular dichroism (CD) spectra, and the apparent affinity binding constants ( K app ) of the porphyrins for CT DNA were determined by using a competition method with ethidium bromide (EB). Intercalation of porphyrin into CT DNA occurred when two phenylpiperazines were introduced at cis position onto the periphery of cationic porphyrin. The photocleavages of pBR322 plasmid DNA by the porphyrins were consistent with the values of K app . With [porphyrin] / [DNA base pairs] ratio increased, the binding mode tended to be outside binding, and the cleavage abilities of the porphyrins varied. In the presence of sodium azide, a quencher of 1 O 2 , the cleavage of DNA by the porphyrin of intercalation was less inhibited.
- Subjects :
- Circular dichroism
Porphyrins
Light
Ultraviolet Rays
Base pair
Stereochemistry
Intercalation (chemistry)
Biophysics
Phenylpiperazine
Binding, Competitive
Biochemistry
Fluorescence
Piperazines
chemistry.chemical_compound
Cations
Ethidium
polycyclic compounds
Animals
Organic chemistry
heterocyclic compounds
Sodium Azide
Molecular Structure
Chimera
Chemistry
Circular Dichroism
Organic Chemistry
Cationic polymerization
DNA
Porphyrin
Intercalating Agents
Kinetics
Spectrometry, Fluorescence
Cattle
Indicators and Reagents
Ethidium bromide
Plasmids
Subjects
Details
- ISSN :
- 03014622
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Biophysical Chemistry
- Accession number :
- edsair.doi.dedup.....1ed848c54777f76041647f5e363e072e