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Desialylation of Spermatozoa and Epithelial Cell Glycocalyx Is a Consequence of Bacterial Infection of the Epididymis
- Source :
- The journal of biological chemistry, 91(34): 17717-17726
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Urinary tract infections caused by uropathogenic Escherichia coli (UPEC) pathovars belong to the most frequent infections in humans. In men, pathogens can also spread to the genital tract via the continuous ductal system, eliciting bacterial prostatitis and/or epididymo-orchitis. Antibiotic treatment usually clears pathogens in acute epididymitis; however, the fertility of patients can be permanently impaired. Because a premature acrosome reaction was observed in an UPEC epididymitis mouse model, and sialidases on the sperm surface are considered to be activated via proteases of the acrosome, we aimed to investigate whether alterations of the sialome of epididymal spermatozoa and surrounding epithelial cells occur during UPEC infection. In UPEC-elicited acute epididymitis in mice, a substantial loss of N-acetylneuraminic acid residues was detected in epididymal spermatozoa and epithelial cells using combined laser microdissection/HPLC-ESI-MS analysis. In support, a substantial reduction of sialic acid residues bound to the surface of spermatozoa was documented in men with a recent history of E. coli-associated epididymitis. In vitro, such an UPEC induced N-acetylneuraminic acid release from human spermatozoa was effectively counteracted by a sialidase inhibitor. These findings strongly suggest a substantial remodeling of the glycocalyx of spermatozoa and epididymal epithelial cells by endogenous sialidases after a premature acrosome reaction during acute epididymitis.
- Subjects :
- Male
0301 basic medicine
Proteases
Acrosome reaction
Neuraminidase
Biology
Glycocalyx
Sialidase
Biochemistry
Microbiology
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Animals
Humans
Uropathogenic Escherichia coli
sialidase
spermatozoa
sialic acid
glycosylation
sperm
glycosylation inhibitor
bacterial pagthogenesis
reproduction
Acrosome
Molecular Biology
Epididymitis
030219 obstetrics & reproductive medicine
urogenital system
Cell Biology
Epididymis
medicine.disease
Spermatozoa
N-Acetylneuraminic Acid
Sialic acid
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
chemistry
Urinary Tract Infections
Immunology
Developmental Biology
Subjects
Details
- ISBN :
- 978-1-77171-772-4
1-77171-772-6 - ISSN :
- 00219258
- ISBNs :
- 9781771717724 and 1771717726
- Volume :
- 291
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....1eb980f455b253de0e05c81643cb6214