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cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust
- Source :
- Insect Molecular Biology. 16:207-219
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- The high population density of insects is often a stress factor. Insects synthesize heat shock proteins (Hsps) in response to the impacts of stress through molecular chaperone activity. Locust solitary and gregarious phases occur at low and high population density, respectively. In this study, we compare the expression profiles of the Hsp genes in the two phases of the migratory locust in an attempt to examine the role of Hsps in adaptation to high density populations. The full length cDNAs of Hsp20.5, 20.6, 20.7, 40, 70 and Hsp90 of the migratory locust were cloned and sequenced. The expressional differentiation of the six Hsps in mRNA levels between solitary and gregarious locusts was observed. Results from real-time PCR indicate that the six Hsps are expressed throughout all developmental stages except in the early stage embryo. The expression levels of the six Hsps were significantly upregulated in gregarious locusts. The expressional variations among certain organs, such as the head, thorax and leg of fifth instar nymphs in gregarious locusts were also higher than those in solitary ones. These observations suggest that population density may be an important factor in determining Hsp expression in the locust.
- Subjects :
- Male
DNA, Complementary
animal structures
media_common.quotation_subject
Molecular Sequence Data
Gene Expression
Locusta migratoria
Genes, Insect
Insect
Biology
Heat shock protein
Gene expression
Genetics
Animals
Amino Acid Sequence
Social Behavior
Molecular Biology
Heat-Shock Proteins
media_common
Population Density
Regulation of gene expression
Ecology
Gene Expression Regulation, Developmental
Embryo
Migratory locust
biology.organism_classification
Adaptation, Physiological
Hsp90
Cell biology
Insect Science
biology.protein
Female
Locust
Subjects
Details
- ISSN :
- 13652583 and 09621075
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Insect Molecular Biology
- Accession number :
- edsair.doi.dedup.....1eb36773cdb4a624144bccdb496eac36