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The RNA Atlas, a single nucleotide resolution map of the human transcriptome

The RNA Atlas, a single nucleotide resolution map of the human transcriptome

Authors :
Francisco Avila Cobos
Jo Vandesompele
Jan Koster
Scott Kuersten
Hua-Sheng Chiu
Robrecht Cannoodt
Ashwin Unnikrishnan
Pavel Sumazin
Justine Nuytens
Marc R. Wilkins
Gary P. Schroth
Katrien Vanderheyden
Thomas B. Hansen
Jasper Anckaert
Steve Lefever
Tom Taghon
Yvan Saeys
Nandan P. Deshpande
Lucia Lorenzi
Jørgen Kjems
Stephen M. Gross
Pieter Mestdagh
Govardhan Anande
Tim De Meyer
Nele Nijs
Tine Goovaerts
Ken R. Bracke
Katleen De Preter
Ting-Wen Chen
Pieter-Jan Volders
Karim Vermaelen
Source :
bioRxiv
Publication Year :
2019
Publisher :
Cold Spring Harbor Laboratory, 2019.

Abstract

The human transcriptome consists of various RNA biotypes including multiple types of non-coding RNAs (ncRNAs). Current ncRNA compendia remain incomplete partially because they are almost exclusively derived from the interrogation of small- and polyadenylated RNAs. Here, we present a more comprehensive atlas of the human transcriptome that is derived from matching polyA-, total-, and small-RNA profiles of a heterogeneous collection of nearly 300 human tissues and cell lines. We report on thousands of novel RNA species across all major RNA biotypes, including a hitherto poorly-cataloged class of non-polyadenylated single-exon long non-coding RNAs. In addition, we exploit intron abundance estimates from total RNA-sequencing to test and verify functional regulation by novel non-coding RNAs. Our study represents a substantial expansion of the current catalogue of human ncRNAs and their regulatory interactions. All data, analyses, and results are available in the R2 web portal and serve as a basis to further explore RNA biology and function.

Details

Language :
English
Database :
OpenAIRE
Journal :
bioRxiv
Accession number :
edsair.doi.dedup.....1eaeb6aacad2ff11458522ade2563d0b
Full Text :
https://doi.org/10.1101/807529