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EFFECTS OF RESVERATROL ON VITRIFIED PORCINE OOCYTES
- Source :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2013 (2013)
- Publication Year :
- 2013
-
Abstract
- Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway. Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapoptotic one. The aim of this study was to determine the effects of R (2 µM) on the apoptotic status of porcine oocytes vitrified by Cryotop method, evaluating phosphatidylserine (PS) exteriorization and caspases activation. R was added during IVM (A); 2 h postwarming incubation (B); vitrification/warming and 2 h postwarming incubation (C); all previous phases (D). Data on PS exteriorization showed, in each treated group, a significantly higher (P<0.05) percentage of live nonapoptotic oocytes as compared with CTR; moreover, the percentage of live apoptotic oocytes was significantly (P<0.05) lower in all R-treated groups relative to CTR. The results on caspase activation showed a tendency to an increase of viable oocytes with inactive caspases in B, C, and D, while a significant (P<0.05) increase in A compared to CTR was recorded. These data demonstrate that R supplementation in various phases of IVM and vitrification/warming procedure can modulate the apoptotic process, improving the resistance of porcine oocytes to cryopreservation-induced damage.
- Subjects :
- Aging
Article Subject
Sus scrofa
Apoptosis
Phosphatidylserines
Resveratrol
VITRIFICATION
Biochemistry
Cryopreservation
Andrology
Enzyme activator
chemistry.chemical_compound
Pig oocytes
Stilbenes
Animals
Apoptotic proce
lcsh:QH573-671
Annexin A5
Incubation
Caspase
RESVERATROL
Apoptotic process
PORCINE OOCYTE
Staining and Labeling
biology
lcsh:Cytology
Cell Biology
General Medicine
Phosphatidylserine
Enzyme Activation
Microscopy, Fluorescence
chemistry
Caspases
Oocytes
biology.protein
Benzimidazoles
Female
Research Article
Propidium
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2013 (2013)
- Accession number :
- edsair.doi.dedup.....1ead6a7b9bf2f82412b48be08de20424