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The neuroprotective effect of eupatilin against ischemia/reperfusion-induced delayed neuronal damage in mice
- Source :
- European Journal of Pharmacology. 689:104-110
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Eupatilin, a pharmacologically active flavone derived from the Artemisia plant species, has been reported to have anti-oxidant, anti-inflammatory, anti-allergic, and anti-tumor activities. In the present study, we investigated whether eupatilin exhibits neuroprotective activities against ischemia/reperfusion-induced delayed neuronal injury in mice. Transient global cerebral ischemia was induced in mice by bilateral common carotid artery occlusion (BCCAO) for 15 min followed by reperfusion for 4 days. Eupatilin (1, 3, or 10 mg/kg, p.o.) was administered immediately after the reperfusion. Histochemical studies showed that eupatilin (10 mg/kg) increased the number of viable cells detected by Nissl staining and decreased the number of degenerating neuronal cells detected by Fluoro-Jade B staining in the hippocampal CA1 region. Western blotting indicated that eupatilin further increased the level of Akt phosphorylation at 8h after BCCAO. Furthermore, wortmannin, a phosphatidylinositol 3-kinase inhibitor, attenuated the eupatilin-induced increase of Akt phosphorylation. In addition, wortmannin completely reversed the eupatilin-induced neuroprotective effects observed at 4 days after reperfusion. These findings suggest that eupatilin is a promising therapeutic agent against global cerebral ischemia-induced neuronal damage and that its neuroprotective effects may be mediated in part by increased Akt phosphorylation.
- Subjects :
- Male
Time Factors
Eupatilin
Ischemia
Pharmacology
Hippocampal formation
Neuroprotection
Brain Ischemia
Wortmannin
Mice
chemistry.chemical_compound
symbols.namesake
Animals
Medicine
Phosphatidylinositol
Flavonoids
Neurons
business.industry
medicine.disease
Blot
Neuroprotective Agents
chemistry
Reperfusion Injury
Anesthesia
Nissl body
symbols
business
Drugs, Chinese Herbal
medicine.drug
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 689
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....1eacde20eb3025247d6abcae6842fa18