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A SPECT-based method for dynamic imaging of the glymphatic system in rats

Authors :
Björn Sigurdsson
Natalie L Hauglund
Tuomas O Lilius
Frida L-H Mogensen
Kristian N Mortensen
Natalie Beschorner
Laura Klinger
Simone L Bærentzen
Marko P Rosenholm
Vladimir Shalgunov
Matthias Herth
Yuki Mori
Maiken Nedergaard
Source :
Sigurdsson, B, Hauglund, N L, Lilius, T O, Mogensen, F L H, Mortensen, K N, Beschorner, N, Klinger, L, Bærentzen, S L, Rosenholm, M P, Shalgunov, V, Herth, M, Mori, Y & Nedergaard, M 2023, ' A SPECT-based method for dynamic imaging of the glymphatic system in rats ', Journal of Cerebral Blood Flow and Metabolism, vol. 43, no. 7, pp. 1153-1165 . https://doi.org/10.1177/0271678X231156982
Publication Year :
2023
Publisher :
SAGE Publications, 2023.

Abstract

The glymphatic system is a brain-wide waste drainage system that promotes cerebrospinal fluid circulation through the brain to remove waste metabolites. Currently, the most common methods for assessing glymphatic function are ex vivo fluorescence microscopy of brain slices, macroscopic cortical imaging, and MRI. While all these methods have been crucial for expanding our understanding of the glymphatic system, new techniques are required to overcome their specific drawbacks. Here, we evaluate SPECT/CT imaging as a tool to assess glymphatic function in different anesthesia-induced brain states using two radiolabeled tracers, [111In]-DTPA and [99mTc]-NanoScan. Using SPECT, we confirmed the existence of brain state-dependent differences in glymphatic flow and we show brain state-dependent differences of CSF flow kinetics and CSF egress to the lymph nodes. We compare SPECT and MRI for imaging glymphatic flow and find that the two imaging modalities show the same overall pattern of CSF flow, but that SPECT was specific across a greater range of tracer concentrations than MRI. Overall, we find that SPECT imaging is a promising tool for imaging the glymphatic system, and that qualities such as high sensitivity and the variety of available tracers make SPECT imaging a good alternative for glymphatic research.

Details

ISSN :
15597016 and 0271678X
Database :
OpenAIRE
Journal :
Journal of Cerebral Blood Flow & Metabolism
Accession number :
edsair.doi.dedup.....1eabd0a8ce22b82df022722572c22944