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Reversing current rectification to improve DNA-sensing sensitivity in conical nanopores

Authors :
Xiu-Hong Cai
Shuo-Hui Cao
Sheng-Lin Cai
Yao-Qun Li
Muhammad Ajmal
Yuan-Yi Wu
Source :
Electrophoresis. 40(16-17)
Publication Year :
2019

Abstract

Herein, we report the ultrasensitive DNA detection through designing an elegant nanopore biosensor as the first case to realize the reversal of current rectification direction for sensing. Attributed to the unique asymmetric structure, the glass conical nanopore exhibits the sensitive response to the surface charge, which can be facilely monitored by ion current rectification curves. In our design, an enzymatic cleavage reaction was employed to alter the surface charge of the nanopore for DNA sensing. The measured ion current rectification was strongly responsive to DNA concentrations, even reaching to the reversed status from the negative ratio (-6.5) to the positive ratio (+16.1). The detectable concentration for DNA was as low as 0.1 fM. This is an ultrasensitive and label-free DNA sensing approach, based on the rectification direction-reversed amplification in a single glass conical nanopore.

Details

ISSN :
15222683
Volume :
40
Issue :
16-17
Database :
OpenAIRE
Journal :
Electrophoresis
Accession number :
edsair.doi.dedup.....1eaa7203a156f613b00cd97ce0ae0ef6