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Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-92806-8⟩, Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-92806-8⟩, Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- G-quadruplexes (G4) are non-canonical secondary structures consisting in stacked tetrads of hydrogen-bonded guanines bases. An essential feature of G4 is their intrinsic polymorphic nature, which is characterized by the equilibrium between several conformations (also called topologies) and the presence of different types of loops with variable lengths. In cells, G4 functions rely on protein or enzymatic factors that recognize and promote or resolve these structures. In order to characterize new G4-dependent mechanisms, extensive researches aimed at identifying new G4 binding proteins. Using G-rich single-stranded oligonucleotides that adopt non-controlled G4 conformations, a large number of G4-binding proteins have been identified in vitro, but their specificity towards G4 topology remained unknown.Constrained G4 structures are biomolecular objects based on the use of a rigid cyclic peptide scaffold as a template for directing the intramolecular assembly of the anchored oligonucleotides into a single and stabilized G4 topology. Here, using various constrained RNA or DNA G4 as baits in human cell extracts, we establish the topology preference of several well-known G4-interacting factors. Moreover, we identify new G4-interacting proteins such as the NELF complex involved in the RNA-Pol II pausing mechanism, and we show that it impacts the clastogenic effect of the G4-ligand pyridostatin.
- Subjects :
- 0301 basic medicine
Science
Oligonucleotides
RNA-binding proteins
RNA-binding protein
Network topology
Topology
DNA-binding protein
Article
NELF complex
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Topology (chemistry)
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Multidisciplinary
Oligonucleotide
RNA
DNA
Cyclic peptide
Protein-protein interaction networks
DNA-Binding Proteins
G-Quadruplexes
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Medicine
RNA Polymerase II
030217 neurology & neurosurgery
HeLa Cells
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-92806-8⟩, Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-92806-8⟩, Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
- Accession number :
- edsair.doi.dedup.....1ea7d762a90d56ace5e8095cfccb74de