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Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi
- Source :
- ACS synthetic biology. 3(12)
- Publication Year :
- 2014
-
Abstract
- The ability to regulate endogenous gene expression is critical in biological research. Existing technologies, such as RNA interference, zinc-finger regulators, transcription-activator-like effectors, and CRISPR-mediated regulation, though proved to be competent in significantly altering expression levels, do not provide a quantitative adjustment of regulation effect. As a solution to this problem, we place CRISPR-mediated interference under the control of blue light: while dCas9 protein is constitutively expressed, guide RNA transcription is regulated by YF1-FixJ-PFixK2, a blue light responding system. With a computer-controlled luminous device, the quantitative relationship between target gene expression and light intensity has been determined. As the light intensifies, the expression level of target gene gradually ascends. This remarkable property enables sensor-CRISPRi to accurately interrogate cellular activities.
- Subjects :
- Light
Effector
Biomedical Engineering
Gene Expression
Endogeny
Bioengineering
General Medicine
Biology
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Molecular biology
Cell biology
Light intensity
Transcription (biology)
RNA interference
Gene expression
Escherichia coli
RNA Interference
Guide RNA
CRISPR-Cas Systems
Blue light
Plasmids
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 3
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- ACS synthetic biology
- Accession number :
- edsair.doi.dedup.....1e8a4fea7ec9fc0e7b64969000967fd8