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A convenient method for saponin isolation in tumour therapy
- Source :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 878(7-8)
- Publication Year :
- 2009
-
Abstract
- Saponinum album (Merck), which is a crude mixture of saponins from Gypsophila paniculata L., was shown to improve the anti cancer therapy when used in vivo in combination with saporin-based targeted toxins. Unfortunately saponinum album cannot be used for further development since Merck has ceased its production in the 1990s. As pure saponins are mandatory for use in medical purposes we developed a convenient method for saponin isolation directly from the roots of Gypsophila paniculata L. The developed method is rapid, cheap and scaling up is also possible. By combining dialysis and HPLC three saponins were isolated in a one-step procedure. Chemical structures of the purified saponins were characterized by extensive one and two-dimensional NMR-spectroscopy and by using ESI-TOF-MS. The biological activities of the purified saponins were also investigated. The method presented herein enabled a rapid and cheap isolation of saponins for tumour therapy.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Gypsophila
Saporin
Cell Survival
Clinical Biochemistry
Molecular Sequence Data
Saponin
Caryophyllaceae
complex mixtures
Biochemistry
Plant Roots
Analytical Chemistry
Cell Line
In vivo
parasitic diseases
Gypsophila paniculata
Humans
Medicinal plants
Nuclear Magnetic Resonance, Biomolecular
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Chromatography
biology
Chemistry
Endothelial Cells
Cell Biology
General Medicine
Saponins
musculoskeletal system
Isolation (microbiology)
biology.organism_classification
Antineoplastic Agents, Phytogenic
carbohydrates (lipids)
Tumour therapy
Carbohydrate Sequence
biology.protein
Dialysis
Subjects
Details
- ISSN :
- 1873376X
- Volume :
- 878
- Issue :
- 7-8
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Accession number :
- edsair.doi.dedup.....1e7c8d9f19047ede531775be20351127