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GC polymorphism detected in human urine by isoelectric focusing and immunoblotting
- Source :
- Forensic science international. 47(1)
- Publication Year :
- 1990
-
Abstract
- Genetic polymorphism of GC (vitamin D-binding protein) in human urine was revealed by isoelectric focusing and immunoblotting on thin-layer polyacrylamide gels containing 2 M urea. Urine samples from 530 unrelated Japanese from the Fukui district, being only 1–2 ml of original urine, were examined, and correct GC typing was achieved by comparison with the results of direct grouping using plasma. Six common and twelve rare phenotypes were observed. The frequencies of the genes were 0.473 for GC ∗ 1F , 0.241 for GC ∗ 1S , 0.254 for GC ∗ 2 , and 0.032 for the total of six rare alleles.
- Subjects :
- Vitamin
medicine.medical_specialty
Immunoblotting
Urine
Immunoelectrophoresis
Biology
Pathology and Forensic Medicine
chemistry.chemical_compound
Gene Frequency
medicine
Humans
Typing
Allele
Alleles
Chromatography
Polymorphism, Genetic
medicine.diagnostic_test
Isoelectric focusing
Vitamin D-Binding Protein
Cytogenetics
Molecular biology
Phenotype
chemistry
Urea
Isoelectric Focusing
Law
Subjects
Details
- ISSN :
- 03790738
- Volume :
- 47
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Forensic science international
- Accession number :
- edsair.doi.dedup.....1e7b29289b11567b753a092a488f6401