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Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA-3 signaling pathway
- Source :
- European Journal of Immunology. 26:610-617
- Publication Year :
- 1996
- Publisher :
- Wiley, 1996.
-
Abstract
- Cultured human endothelial cells (EC) increase CD40 ligand expression on polyclonally activated human peripheral blood CD4+ helper T cells compared to T cells activated in the absence of accessory cells or in the presence of peripheral blood adherent cells or B cells. Induction of CD40 ligand expression appears to be biphasic with early induction observable at 6 h and later induction observable at 24 h. EC cause T cells to increase CD40 ligand expression during the early phase at 6 h after activation. CD40 ligand expression is restricted to the CD4+ helper T cell subset of the peripheral blood T cells, even when EC are present. Blocking monoclonal antibodies to co-stimulatory molecules on EC and T cells indicate that the CD2/LFA-3 pathway, which also contributes to induction of augmented interleukin-2 (IL-2) secretion, is involved in EC-induced upregulation of CD40 ligand. Exogenous IL-2 can also increase CD40 ligand expression. However, increased IL-2 secretion in the presence of EC can not fully account for endothelial-induced CD40 ligand up-regulation as (1) the effect of exogenous IL-2 is greater at 24 h than at 6 h, whereas the opposite is true for EC; (2) the effect of saturating levels of IL-2 is considerably smaller than that of EC; and (3) blocking of IL-2 receptors does not fully inhibit endothelial effects on CD40 ligand expression. We conclude that EC provide unique co-stimulatory signals that affect the phenotype of activated CD4+ T cells.
- Subjects :
- Adult
Antigens, Differentiation, T-Lymphocyte
CD4-Positive T-Lymphocytes
CD40 Ligand
Immunology
CD2 Antigens
Cell Communication
Biology
Ligands
Lymphocyte Activation
Interleukin 21
Cell Adhesion
Humans
Immunology and Allergy
Cytotoxic T cell
IL-2 receptor
Phytohemagglutinins
Antigen-presenting cell
Receptor
Cells, Cultured
B-Lymphocytes
Membrane Glycoproteins
CD40
ZAP70
CD58 Antigens
Natural killer T cell
Molecular biology
Up-Regulation
Cell biology
biology.protein
Interleukin-2
Endothelium, Vascular
Signal Transduction
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....1e6ab3d6a842463af8a45cb7649fa973