Back to Search
Start Over
Aspirin suppresses the abnormal lipid metabolism in liver cancer cells via disrupting an NFκB-ACSL1 signaling
- Source :
- Biochemical and Biophysical Research Communications. 486:827-832
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Abnormal lipid metabolism is a hallmark of tumorigenesis. Hence, the alterations of metabolism enhance the development of hepatocellular carcinoma (HCC). Aspirin is able to inhibit the growth of cancers through targeting nuclear factor κB (NF-κB). However, the role of aspirin in disrupting abnormal lipid metabolism in HCC remains poorly understood. In this study, we report that aspirin can suppress the abnormal lipid metabolism of HCC cells through inhibiting acyl-CoA synthetase long-chain family member 1 (ACSL1), a lipid metabolism-related enzyme. Interestingly, oil red O staining showed that aspirin suppressed lipogenesis in HepG2 cells and Huh7 cells in a dose-dependent manner. In addition, aspirin attenuated the levels of triglyceride and cholesterol in the cells, respectively. Strikingly, we identified that aspirin was able to down-regulate ACSL1 at the levels of mRNA and protein. Moreover, we validated that aspirin decreased the nuclear levels of NF-κB in HepG2 cells. Mechanically, PDTC, an inhibitor of NF-κB, could down-regulate ACSL1 at the levels of mRNA and protein in the cells. Functionally, PDTC reduced the levels of lipid droplets, triglyceride and cholesterol in HepG2 cells. Thus, we conclude that aspirin suppresses the abnormal lipid metabolism in HCC cells via disrupting an NFκB-ACSL1 signaling. Our finding provides new insights into the mechanism by which aspirin inhibits abnormal lipid metabolism of HCC. Therapeutically, aspirin is potentially available for HCC through controlling abnormal lipid metabolism.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Proline
Biophysics
Biology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Thiocarbamates
Cell Line, Tumor
Lipid droplet
Internal medicine
Coenzyme A Ligases
medicine
Humans
Oil Red O
RNA, Messenger
Molecular Biology
Triglycerides
Aspirin
Dose-Response Relationship, Drug
Cholesterol
Anti-Inflammatory Agents, Non-Steroidal
NF-kappa B
Lipid metabolism
Hep G2 Cells
Lipid Droplets
Cell Biology
Lipid Metabolism
NFKB1
digestive system diseases
Gene Expression Regulation, Neoplastic
030104 developmental biology
Endocrinology
chemistry
030220 oncology & carcinogenesis
Lipogenesis
Cancer research
Signal transduction
Azo Compounds
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 486
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....1e69898abd77fbdfb7fd847887a2fab9