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Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 2, p e0172139 (2017)
- Publication Year :
- 2016
-
Abstract
- Detection of histidine-rich protein 2 (HRP2) from the malaria parasite Plasmodium falciparum provides evidence for active or recent infection, and is utilized for both diagnostic and surveillance purposes, but current laboratory immunoassays for HRP2 are hindered by low sensitivities and high costs. Here we present a new HRP2 immunoassay based on antigen capture through a bead-based system capable of detecting HRP2 at sub-picogram levels. The assay is highly specific and cost-effective, allowing fast processing and screening of large numbers of samples. We utilized the assay to assess results of HRP2-based rapid diagnostic tests (RDTs) in different P. falciparum transmission settings, generating estimates for true performance in the field. Through this method of external validation, HRP2 RDTs were found to perform well in the high-endemic areas of Mozambique and Angola with 86.4% and 73.9% of persons with HRP2 in their blood testing positive by RDTs, respectively, and false-positive rates of 4.3% and 0.5%. However, in the low-endemic setting of Haiti, only 14.5% of persons found to be HRP2 positive by the bead assay were RDT positive. Additionally, 62.5% of Haitians showing a positive RDT test had no detectable HRP2 by the bead assay, likely indicating that these were false positive tests. In addition to RDT validation, HRP2 biomass was assessed for the populations in these different settings, and may provide an additional metric by which to estimate P. falciparum transmission intensity and measure the impact of interventions.
- Subjects :
- 0301 basic medicine
Plasmodium
Endemic Diseases
Quantitative Parasitology
Protozoan Proteins
lcsh:Medicine
Parasitemia
Biochemistry
Antigen capture
Geographical Locations
0302 clinical medicine
Medicine and Health Sciences
Medicine
Malaria, Falciparum
Enzyme-Linked Immunoassays
Blood testing
lcsh:Science
Child
Mozambique
Aged, 80 and over
Immunoassay
Multidisciplinary
biology
medicine.diagnostic_test
Diagnostic test
Middle Aged
Recombinant Proteins
Child, Preschool
Research Article
Adult
Adolescent
030231 tropical medicine
030106 microbiology
Plasmodium falciparum
Antigens, Protozoan
Research and Analysis Methods
Sensitivity and Specificity
Host-Parasite Interactions
03 medical and health sciences
Young Adult
parasitic diseases
Parasite Groups
Parasitic Diseases
Humans
Amino Acid Sequence
Immunoassays
Aged
business.industry
Diagnostic Tests, Routine
lcsh:R
External validation
Infant
Reproducibility of Results
Biology and Life Sciences
Proteins
medicine.disease
biology.organism_classification
Tropical Diseases
Health Surveys
Haiti
Malaria
Cross-Sectional Studies
Angola
Immunology
People and Places
Africa
Immunologic Techniques
lcsh:Q
Parasitology
business
Apicomplexa
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....1e4eba040d6e74dd9a581de9b7738ef4