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Mechanism of Intramolecular Photostabilization in Self-Healing Cyanine Fluorophores
- Source :
- Chemphyschem, 14(18), 4084-4093. WILEY-V C H VERLAG GMBH, ChemPhysChem
- Publication Year :
- 2013
- Publisher :
- WILEY-V C H VERLAG GMBH, 2013.
-
Abstract
- Organic fluorophores, which are popular labels for microscopy applications, intrinsically suffer from transient and irreversible excursions to dark-states. An alternative to adding photostabilizers at high concentrations to the imaging buffer relies on the direct linkage to the fluorophore. However, the working principles of this approach are not yet fully understood. In this contribution, we investigate the mechanism of intramolecular photostabilization in self-healing cyanines, in which photodamage is automatically repaired. Experimental evidence is provided to demonstrate that a single photostabilizer, that is, the vitamin E derivative Trolox, efficiently heals the cyanine fluorophore Cy5 in the absence of any photostabilizers in solution. A plausible mechanism is that Trolox interacts with the fluorophore through intramolecular quenching of triplet-related dark-states, which is a mechanism that appears to be common for both triplet-state quenchers (cyclooctatetraene) and redox-active compounds (Trolox, ascorbic acid, methylviologen). Additionally, the influence of solution-additives, such as cysteamine and procatechuic acid, on the self-healing process are studied. The results suggest the potential applicability of self-healing fluorophores in stochastic optical reconstruction microscopy (STORM) with optical super-resolution. The presented data contributes to an improved understanding of the mechanism involved in intramolecular photostabilization and has high relevance for the future development of self-healing fluorophores, including their applications in various research fields.
- Subjects :
- STABILIZATION
Fluorophore
ORGANIC FLUOROPHORES
photostabilizer
single-molecule studies
010402 general chemistry
Photochemistry
01 natural sciences
OPTICAL RECONSTRUCTION MICROSCOPY
03 medical and health sciences
chemistry.chemical_compound
BLINKING
self-healing
Physical and Theoretical Chemistry
Cyanine
FLUORESCENT-PROBES
OXIDIZING SYSTEM
030304 developmental biology
0303 health sciences
Quenching (fluorescence)
cyanines
SPECTROSCOPY
Chemistry
Ascorbic acid
Fluorescence
Atomic and Molecular Physics, and Optics
LASER-DYES
0104 chemical sciences
Intramolecular force
TRIPLET
Trolox
fluorescence
SUPERRESOLUTION MICROSCOPY
Derivative (chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 14394235
- Volume :
- 14
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Chemphyschem
- Accession number :
- edsair.doi.dedup.....1e390aeb7e0eea9ec573e3e59c0fb047