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Preparation and properties of 2′(or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate
Preparation and properties of 2′(or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate
- Source :
- Biochimica et Biophysica Acta (BBA) - General Subjects. 320:635-647
- Publication Year :
- 1973
- Publisher :
- Elsevier BV, 1973.
-
Abstract
- An analog of adenosine triphosphate, 2′(or 3′)-O-(2,4,6-trinitrophenyl)adenosine 5′-triphosphate (TNP-ATP), was synthesized as a reporter-labeled substrate of heavy meromyosin ATPase. TNP-ATP was hydrolyzed by heavy meromyosin in the presence of CaCl2 MgCl2 or EDTA. TNP-ATP had absorption maxima at 259 nm, 408 nm and 470 nm at neutral pH. When bound to heavy meromyosin, TNP-ATP underwent the characteristic spectral shift. The difference spectrum resulting from the binding of TNP-ATP to heavy meromyosin at pH 8.0 had positive peaks at 415 nm and 518 nm, and a negative trough at 458 nm. The difference spectrum due to the binding of 2′(or 3′)-O-(2,4,6-trinitrophenyl)adenosine (TNP-adenosine) to heavy meromyosin had small positive peaks at 420 nm and 495 nm. This difference spectrum was similar to that of TNP-ATP or TNP-adenosine produced by 20% (v/v) ethyleneglycol perturbation. The positive peak at 495 nm in the difference spectrum due to the binding of TNP-adenosine to heavy meromyosin shifted toward 505 nm, when pyrophosphate or ATP was added to the reaction mixture. These results suggest that the difference spectrum of TNP-ATP due to the interaction with heavy meromyosin arises not only from the binding of the chromophoric portion of the TNP-ATP molecule but also from that of the phosphate portion.
- Subjects :
- Magnetic Resonance Spectroscopy
Spectrophotometry, Infrared
Chromatography, Paper
Stereochemistry
ATPase
Biophysics
chemical and pharmacologic phenomena
macromolecular substances
Biochemistry
Pyrophosphate
Chromatography, DEAE-Cellulose
Structure-Activity Relationship
chemistry.chemical_compound
Hydrolysis
Adenosine Triphosphate
medicine
Molecule
Molecular Biology
Nitrobenzenes
Adenosine Triphosphatases
biology
Heavy meromyosin
Chemistry
Benzenesulfonates
Myosin Subfragments
hemic and immune systems
Hydrogen-Ion Concentration
Phosphate
Adenosine
eye diseases
Kinetics
Crystallography
Spectrophotometry
Chromatography, Gel
biology.protein
Spectrophotometry, Ultraviolet
tissues
Adenosine triphosphate
medicine.drug
Subjects
Details
- ISSN :
- 03044165
- Volume :
- 320
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Accession number :
- edsair.doi.dedup.....1e34678c15a312a4fa0fc03e1356ac65
- Full Text :
- https://doi.org/10.1016/0304-4165(73)90143-8