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Alteration of expression or phosphorylation status of tob, a novel tumor suppressor gene product, is an early event in lung cancer
- Source :
- Cancer Letters. 202:71-79
- Publication Year :
- 2003
- Publisher :
- Elsevier BV, 2003.
-
Abstract
- Tob is a member of the Tob/BTG family, a novel class of anti-proliferative proteins. To investigate the involvement of tob as a tumor suppressor gene in human lung cancer, we analyzed the expression of tob mRNA and protein in lung cancer tissue and adjacent normal lung tissue. Immunohistochemical analysis using anti-Tob antibody showed decreased expression of Tob in 72% (31/43) of lung cancer tissues. Furthermore, 95% (19/20) of squamous cell carcinoma patients showed an apparent decrease in Tob in cancer tissues, associated with smoking status. The phosphorylated form of Tob, an inactive form of Tob, was detected in 76% (16/21) of cancer tissues of adenocarcinoma patients, but not in normal alveolar epithelial cells. Either a decrease in Tob expression or an accumulation of phosphorylated Tob was observed from early clinical stages, even in bronchial dysplasia, a premalignant lesion of squamous cell carcinoma. The above findings suggest that the disruption of anti-proliferative Tob plays a distinct part in the early stage of lung carcinogenesis.
- Subjects :
- Male
Cancer Research
Lung Neoplasms
Tumor suppressor gene
Bronchi
Adenocarcinoma
Biology
medicine.disease_cause
Immunoenzyme Techniques
Gene expression
Carcinoma
medicine
Humans
Genes, Tumor Suppressor
RNA, Messenger
RNA, Neoplasm
Phosphorylation
Lung cancer
Aged
Neoplasm Staging
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Proteins
Intracellular Signaling Peptides and Proteins
Cancer
Epithelial Cells
medicine.disease
Radiography
Oncology
Immunoglobulin G
Carcinoma, Squamous Cell
Cancer research
Immunohistochemistry
Female
Carrier Proteins
Carcinogenesis
Precancerous Conditions
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 202
- Database :
- OpenAIRE
- Journal :
- Cancer Letters
- Accession number :
- edsair.doi.dedup.....1e26e563855346bc5efa217a917faff0