Back to Search
Start Over
A simple and rapid immunochromatography test based on readily available filter paper modified with chitosan to screen for 13 sulfonamides in milk
- Source :
- Journal of Dairy Science. 104:126-133
- Publication Year :
- 2021
- Publisher :
- American Dairy Science Association, 2021.
-
Abstract
- In this study, we developed a novel, simple, rapid, and low-cost colloidal gold-based immunochromatography method, with filter paper replacing nitrocellulose membrane as the substrate. To obtain adequately immobilized protein, chitosan was used to functionalize the filter paper. After conditions and parameters were optimized, the novel immunochromatography method was applied for detection of sulfonamide residues in milk. Quantitative detection was accomplished using a smartphone and Photoshop software (Adobe Inc., San Jose, CA), allowing us to screen 13 sulfonamides with a limit of detection ranging from 0.42 to 8.64 μg/L and recovery ranging from 88.2 to 116.9% in milk. The proposed novel method performed similarly to the conventional method that uses a nitrocellulose membrane as the transport medium, and it had lower cost and better usability because of the inexpensive and easily available filter paper.
- Subjects :
- Paper
Immobilized protein
Materials science
Gold Colloid
Chromatography, Affinity
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Transport medium
Genetics
Animals
030304 developmental biology
Detection limit
Sulfonamides
Vaccines
0303 health sciences
Chromatography
Filter paper
0402 animal and dairy science
04 agricultural and veterinary sciences
040201 dairy & animal science
Milk
chemistry
Colloidal gold
Animal Science and Zoology
Lower cost
Nitrocellulose
Filtration
Food Science
Subjects
Details
- ISSN :
- 00220302
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Journal of Dairy Science
- Accession number :
- edsair.doi.dedup.....1e218f144b411235645c94f85bf6dc98