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Propofol Represses Cell Growth and Metastasis by Modulating the Circular RNA Non-SMC Condensin I Complex Subunit G/MicroRNA-200a-3p/RAB5A Axis in Glioma
- Source :
- World Neurosurgery. 153:e46-e58
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Background Glioma is a common primary intracranial tumor, with high infiltration and aggression. Propofol (Pro) is associated with growth and metastasis in glioma. Meanwhile, circular RNA non-SMC condensin I complex subunit G (circNCAPG; hsa_circ_0007244) has been reported to be upregulated in glioma. This study explored the role and mechanism of circNCAPG in Pro-induced glioma progression. Methods Cell viability was determined by cell counting kit-8 assay. Levels of circNCAPG, microRNA-200a-3p (miR-200a-3p), and member RAS oncogene family (RAB5A) were detected by real-time quantitative polymerase chain reaction. Colony number, apoptosis, migration, and invasion were analyzed by colony formation, flow cytometry, wound healing, and transwell assays. Matrix metallopeptidase 2, matrix metallopeptidase 9, and RAB5A protein levels were detected by Western blot assay. The binding relationship between miR-200a-3p and circNCAPG or RAB5A was predicted by starBase 2.0 and then verified by a dual-luciferase reporter and RNA immunoprecipitation assays. The biological roles of circNCAPG and Pro on glioma tumor growth were examined by the xenograft tumor model in vivo. Results Expression of circNCAPG and RAB5A was upregulated, and miR-200a-3p was decreased in glioma tissues and cells, while their expression presented an opposite trend in Pro-treated glioma cells. Moreover, circNCAPG overexpression could abolish Pro-mediated proliferation, apoptosis, migration, and invasion in glioma cells in vitro. Mechanically, circNCAPG could regulate RAB5A expression by sponging miR-200a-3p. Pro blocked glioma tumor growth in vivo by modulating circNCAPG. Conclusions Pro could inhibit glioma cell growth and metastasis through the circNCAPG/miR-200a-3p/RAB5A axis, providing a promising therapeutic strategy for glioma treatment.
- Subjects :
- Adult
Male
Cell Survival
Apoptosis
Cell Cycle Proteins
Real-Time Polymerase Chain Reaction
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Glioma
microRNA
medicine
Humans
Neoplasm Invasiveness
Viability assay
Propofol
Tumor Stem Cell Assay
Cell Proliferation
rab5 GTP-Binding Proteins
medicine.diagnostic_test
Oncogene
Brain Neoplasms
Cell growth
business.industry
RNA, Circular
Middle Aged
medicine.disease
Condensin I complex
MicroRNAs
Matrix Metalloproteinase 9
030220 oncology & carcinogenesis
Cancer research
Matrix Metalloproteinase 2
Female
Surgery
Neurology (clinical)
business
Anesthetics, Intravenous
Neoplasm Transplantation
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18788750
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- World Neurosurgery
- Accession number :
- edsair.doi.dedup.....1e1e43a8b293ec7918fcd630df818a4c