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Nuclear Factor (NF)-κB2 (p100/p52) Is Required for Normal Splenic Microarchitecture and B Cell–mediated Immune Responses
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1998
- Publisher :
- Rockefeller University Press, 1998.
-
Abstract
- The nfkb2 gene is a member of the Rel/NF-kappa B family of transcription factors. COOH-terminal deletions and rearrangements of this gene have been associated with the development of human cutaneous T cell lymphomas, chronic lymphocytic leukemias, and multiple myelomas. To further investigate the function of NF-kappa B2, we have generated mutant mice carrying a germline mutation of the nfkb2 gene by homologous recombination. NF-kappa B2-deficient mice showed a marked reduction in the B cell compartment in spleen, bone marrow, and lymph nodes. Moreover, spleen and lymph nodes of mutant mice presented an altered architecture, characterized by diffuse, irregular B cell areas and the absence of discrete perifollicular marginal and mantle zones; the formation of secondary germinal centers in spleen was also impaired. Proliferation of NF-kappa B2-deficient B cells was moderately reduced in response to lipopolysaccharide, anti-IgD-dextran, and CD40, but maturation and immunoglobulin switching were normal. However, nfkb2 (-/-) animals presented a deficient immunological response to T cell-dependent and -independent antigens. These findings indicate an important role of NF-kappa B2 in the maintenance of the peripheral B cell population, humoral responses, and normal spleen architecture.
- Subjects :
- Lipopolysaccharides
T cell
Immunology
B-Lymphocyte Subsets
Immunoglobulins
Receptors, Antigen, B-Cell
Bone Marrow Cells
Spleen
Biology
Lymphocyte Activation
Article
Epitopes
Mice
Immune system
NF-kappa B p52 Subunit
Lymphopenia
medicine
Animals
Immunology and Allergy
CD40 Antigens
B cell
Mice, Knockout
Immunity, Cellular
Mice, Inbred ICR
CD40
NF-kappa B
Germinal center
Articles
Germinal Center
NFKB1
Molecular biology
Mice, Inbred C57BL
Mutagenesis, Insertional
medicine.anatomical_structure
biology.protein
Female
Lymph Nodes
Antibody
Signal Transduction
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....1e136677334c9550d4771fa47210327b