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Microbial Synthesis of Folate Polyglutamates from Labelled Precursors
- Source :
- Journal of General Microbiology. 84:163-172
- Publication Year :
- 1974
- Publisher :
- Microbiology Society, 1974.
-
Abstract
- SUMMARY: The uptake and synthesis of folate polyglutamates from [3H]pteroylglutamic acid and [14C]p-aminobenzoic acid was studied in Lactobacillus casei, Streptococcus faecalis, L. plantarum and Escherichia coli. The analytical techniques were based on oxidative or hydrolytic degradation of the folate compounds followed by chromatographic separation of the resulting mixtures of pteroylglutamate derivatives or p-aminobenzoylpoly-γ-l-glutamates and comparison with appropriate synthetic standards. All four organisms synthesized numerous polyglutamate derivatives from the precursors provided. The large majority (85 to 95%) of folates of lactobacilli were polyglutamates with more than seven glutamyl residues; L. casei formed several such unidentified long-chain components. In contrast, E. coli and particularly S. faecalis synthesized mainly polyglutamates with seven or less glutamate residues; tetra-, hexa- and heptaglutamates were tentatively identified in E. coli, and tri-, tetra- and pentaglutamates in S. faecalis. While L. casei appeared to synthesize a mixture of formyl- and methyltetrahydro derivatives, formyltetrahydro derivatives predominated in E. coli and S. faecalis.
- Subjects :
- Lactobacillus casei
Oxidative phosphorylation
Tritium
medicine.disease_cause
Microbiology
Chromatography, DEAE-Cellulose
Folic Acid
Glutamates
Species Specificity
Enterococcus faecalis
Escherichia coli
medicine
Aminobenzoates
Carbon Radioisotopes
Folate Polyglutamates
Polyglutamate
biology
Chemistry
Streptococcus
food and beverages
Chromatography, Ion Exchange
HEXA
biology.organism_classification
Lactobacillus
Lacticaseibacillus casei
Biochemistry
Tetra
Subjects
Details
- ISSN :
- 00221287
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Journal of General Microbiology
- Accession number :
- edsair.doi.dedup.....1e0c6af04ba05932a42262768f544dd9