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Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 51(8)
- Publication Year :
- 2003
-
Abstract
- VIP36 (36-kD vesicular integral membrane protein), originally purified from Madin-Darby canine kidney (MDCK) epithelial cells, belongs to a family of animal lectins and may act as a cargo receptor. To understand its role in secretory processes, we performed morphological analysis of the rat parotid gland. Immunoelectron microscopy provided evidence that endogenous VIP36 is localized in the trans-Golgi network, on immature granules, and on mature secretory granules in acinar cells. Double-staining immunofluorescence experiments confirmed that VIP36 and amylase co-localized in the apical regions of the acinar cells. This is the first study to demonstrate that endogenous VIP36 is involved in the post-Golgi secretory pathway, suggesting that VIP36 plays a role in trafficking and sorting of secretory and/or membrane proteins during granule formation.
- Subjects :
- 0301 basic medicine
Male
Histology
Immunoelectron microscopy
Immunoblotting
Golgi Apparatus
03 medical and health sciences
symbols.namesake
Chlorocebus aethiops
Tumor Cells, Cultured
Animals
Parotid Gland
Rats, Wistar
Microscopy, Immunoelectron
Integral membrane protein
Vero Cells
Secretory pathway
Microscopy, Confocal
030102 biochemistry & molecular biology
biology
Secretory Vesicles
Granule (cell biology)
Cell Membrane
Chromogranin A
Membrane Proteins
Membrane Transport Proteins
Golgi apparatus
Cell biology
Rats
030104 developmental biology
Secretory protein
Mannose-Binding Lectins
Membrane protein
Microscopy, Fluorescence
symbols
biology.protein
Anatomy
Carrier Proteins
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 51
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....1e0c3134787f6c3b18d4bab5f56bffc9