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Progress toward an enhanced vaccine: Eight marked attenuated viruses to porcine reproductive and respiratory disease virus
- Source :
- Virology. 516:30-37
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Recombinant viruses of strain Ingelvac® PRRS porcine reproductive and respiratory syndrome virus (PRRSV) modified live virus vaccine were produced with two individual small in-frame deletions in nonstructural protein 2 (nsp2; Δ23 and Δ87) and also the same deletions supplanted with foreign tags (Δ23-V5, Δ23-FLAG, Δ23-S, Δ87-V5, Δ87-FLAG, Δ87-S). The viruses, but one (Δ87-FLAG), were stable for 10 passages and showed minimal effects on in vitro growth. Northern hybridization showed that the Δ23-tagged probe detected intracellular viral genome RNA as well as shorter RNAs that may represent heteroclite species, while the Δ87-tagged probe detected predominantly only genome length RNAs. When the tagged viruses were used to probe nsp2 protein in infected cells, perinuclear localization similar to native nsp2 was seen. Dual infection of Δ23-S and Δ87-S viruses allowed some discrimination of individual tagged nsp2 protein, facilitating future research. The mutants could potentially also be used to differentiate infected from vaccinated animals.
- Subjects :
- 0301 basic medicine
Swine
viruses
Mutant
Porcine Reproductive and Respiratory Syndrome
Viral Nonstructural Proteins
Antibodies, Viral
Vaccines, Attenuated
Genome
Virus
law.invention
03 medical and health sciences
FLAG-tag
law
Virology
Animals
Porcine respiratory and reproductive syndrome virus
biology
RNA
Viral Vaccines
Porcine reproductive and respiratory syndrome virus
biology.organism_classification
030104 developmental biology
Recombinant DNA
Intracellular
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 516
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....1e086bc84d14aef3c0e7b95f8c9f04de