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Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation
- Source :
- Journal of tissue engineering and regenerative medicineREFERENCES. 15(11)
- Publication Year :
- 2021
-
Abstract
- Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
- Subjects :
- Proteomics
Angiogenesis
Cell Survival
Neurogenesis
Biomedical Engineering
Medicine (miscellaneous)
Neovascularization, Physiologic
Umbilical vein
Biomaterials
Extracellular matrix
Meninges
Extracellular
medicine
Animals
Humans
Spinal Meninges
Cell Proliferation
Glycosaminoglycans
Decellularization
Tissue Engineering
Chemistry
Mesenchymal stem cell
Temperature
Cell Differentiation
Hydrogels
Mesenchymal Stem Cells
DNA
Cell biology
Extracellular Matrix
Drug Combinations
Hydroxyproline
Kinetics
medicine.anatomical_structure
Spinal Cord
Wettability
Cattle
Proteoglycans
Collagen
Laminin
Rheology
Porosity
Subjects
Details
- ISSN :
- 19327005
- Volume :
- 15
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of tissue engineering and regenerative medicineREFERENCES
- Accession number :
- edsair.doi.dedup.....1dfc54b9e09501f0b394fc47dd0909de