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RNA-Dependent RNA Polymerases of Both Virulent and Benign Rabbit Caliciviruses Induce Striking Rearrangement of Golgi Membranes
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 1, p e0169913 (2017)
- Publication Year :
- 2016
-
Abstract
- The extremely pathogenic Rabbit haemorrhagic disease virus (RHDV) and the completely benign Rabbit calicivirus (RCV) are closely related members of the genus Lagovirus (family Caliciviridae). The molecular mechanisms that determine the dramatic difference in virulence are unknown, but indirect evidence suggests that different properties of their RNA-dependent RNA polymerases (RdRps) may at least partially be responsible for the contrasting phenotypes. Here we report that the unusual ability of the RHDV RdRp to induce a striking rearrangement of the Golgi network is not specific to RHDV, but a common feature of virulent and benign rabbit caliciviruses alike. Expression of rabbit calicivirus RdRps induced a redistribution of both cis/medial and medial/trans Golgi membrane markers, but not that of an endoplasmic reticulum membrane marker. Inactivating mutations in the conserved GDD motif did not abolish the ability of RHDV RdRp to rearrange the Golgi network, suggesting that polymerase activity and metal co-factors are not required for this function. Finally, we discuss possible implications of RdRp-induced membrane rearrangements on virus replication and host immune responses.
- Subjects :
- 0301 basic medicine
RNA viruses
Viral Diseases
Hemorrhagic Disease Virus, Rabbit
viruses
Amino Acid Motifs
lcsh:Medicine
Golgi Apparatus
Bioinformatics
Virus Replication
Pathology and Laboratory Medicine
Biochemistry
Polymerases
Rabbit haemorrhagic disease
Medicine and Health Sciences
lcsh:Science
Polymerase
Mammals
Staining
Multidisciplinary
Secretory Pathway
Virulence
Cell Staining
Animal Models
Recombinant Proteins
Membrane Staining
Infectious Diseases
Experimental Organism Systems
Medical Microbiology
Cell Processes
Viral Pathogens
Viruses
Vertebrates
symbols
Rabbits
Pathogens
Cellular Structures and Organelles
Research Article
Biology
Research and Analysis Methods
Microbiology
Virus
Caliciviruses
Cell Line
03 medical and health sciences
symbols.namesake
Viral Proteins
DNA-binding proteins
Animals
Microbial Pathogens
Endoplasmic reticulum membrane
Biology and life sciences
lcsh:R
Organisms
RNA
Proteins
Calicivirus Infection
Cell Biology
Golgi apparatus
biology.organism_classification
RNA-Dependent RNA Polymerase
Virology
030104 developmental biology
Viral replication
Specimen Preparation and Treatment
Amniotes
biology.protein
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....1df448bdee8025872fdc94be7779fa49