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Cystic fibrosis transmembrane conductance regulator (CFTR) expression in human platelets: impact on mediators and mechanisms of the inflammatory response

Authors :
Assunta Pandolfi
Luisa Pieroni
Nicola Martelli
Luca Battistini
Daniela F. Angelini
Antonio Recchiuti
Iole Robuffo
Stefano Manarini
Maria Lucia Furnari
Tiziana Pensabene
Giovanna Petrucci
Sara Di Silvestre
Stefano Lancellotti
Francesca Pardo
Mario R. Romano
Virgilio Evangelista
Serena Quattrucci
Domenico Mattoscio
Raimondo De Cristofaro
Giovanni Davì
Bianca Rocca
Source :
The FASEB Journal. 24:3970-3980
Publication Year :
2010
Publisher :
Wiley, 2010.

Abstract

Inflammatory lung disease is a primary cause of morbidity and mortality in cystic fibrosis (CF). Mechanisms of unresolved acute inflammation in CF are not completely known, although the involvement of cystic fibrosis transmembrane conductance regulator (CFTR) in nonrespiratory cells is emerging. Here we examined CFTR expression and function in human platelets (PLTs) and found that they express a biologically active CFTR. CFTR blockade gave an ∼50% reduction in lipoxin A(4) (LXA(4)) formation during PLT/polymorphonuclear leukocytes (PMN) coincubations by inhibiting the lipoxin synthase activity of PLT 12-lipoxygenase. PLTs from CF patients generated ∼40% less LXA(4) compared to healthy subject PLTs. CFTR inhibition increased PLT-dependent PMN viability (33.0±5.7 vs. 61.2±8.2%; P=0.033), suppressed nitric oxide generation (0.23±0.04 vs. 0.11±0.002 pmol/10(8) PLTs; P=0.004), while reducing AKT (1.02±0.12 vs. 0.71±0.007 U; P=0.04), and increasing p38 MAPK phosphorylation (0.650±0.09 vs. 1.04±0.24 U; P=0.03). Taken together, these findings indicate that PLTs from CF patients are affected by the molecular defect of CFTR. Moreover, this CF PLT abnormality may explain the failure of resolution in CF.

Details

ISSN :
15306860 and 08926638
Volume :
24
Database :
OpenAIRE
Journal :
The FASEB Journal
Accession number :
edsair.doi.dedup.....1da84607ac39b5c1a6332e211abfdeff