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Back to the past: deciphering cultural heritage secrets by protein identification
- Source :
- Applied microbiology and biotechnology 102 (2018): 5445–5455. doi:10.1007/s00253-018-8963-z, info:cnr-pdr/source/autori:Giuffrida, Maria Gabriella; Mazzoli, Roberto; Pessione, Enrica/titolo:Back to the past: deciphering cultural heritage secrets by protein identification/doi:10.1007%2Fs00253-018-8963-z/rivista:Applied microbiology and biotechnology/anno:2018/pagina_da:5445/pagina_a:5455/intervallo_pagine:5445–5455/volume:102
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The present review article reports the most innovative methods to detect proteins in historical and archeological samples as well as to characterize proteins used as binders in artworks. Difficulties to ascribe proteins to a certain animal species are often due to post-translational modifications originated by chemical or microbial deterioration during aging. Combining different techniques such as peptide mass fingerprinting and tandem mass spectrometry can solve some of these problems and also allow discrimination between taxonomically related species like sheep and goat. The most studied proteins in bones and textile samples are osteocalcin, collagen and keratin, whereas egg yolk and white proteins, casein and collagen are the most relevant for binders used in old paintings. With the suitable approaches (immune-based methods, DOT-blot, etcaEuro broken vertical bar) it is also possible to obtain in situ characterization or analyze the samples directly in the museum laboratories, with the advantage of avoiding artwork damage and expensive external commitments. Recent cutting-edge strategies allowed detection of proteinaceous infection markers that, for instance, were used to establish the cause of death of old Inca mummies and also proved the presence of Yersinia pestis in old documents dating from the period in 17th century in which the plague ravaged Europe.
- Subjects :
- Paper
0301 basic medicine
Archeology
Biology
Infections
01 natural sciences
Applied Microbiology and Biotechnology
Bone and Bones
03 medical and health sciences
Peptide mass fingerprinting
Animals
Animal species
Mass spectrometry
Textiles
010401 analytical chemistry
Caseins
Proteins
General Medicine
Wall paintings
0104 chemical sciences
Cultural heritage
030104 developmental biology
Archaeology
Keratin
Evolutionary biology
Egg yolk
Biotechnology
Paintings
Protein identification
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....1d8b36c47418f081dab619691cf4194c