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A genetic screen for terminator function in yeast identifies a role for a new functional domain in termination factor Nab3
- Source :
- Nucleic Acids Research
- Publication Year :
- 2012
- Publisher :
- Oxford University Press (OUP), 2012.
-
Abstract
- The yeast IMD2 gene encodes an enzyme involved in GTP synthesis. Its expression is controlled by guanine nucleotides through a set of alternate start sites and an intervening transcriptional terminator. In the off state, transcription results in a short non-coding RNA that starts upstream of the gene. Transcription terminates via the Nrd1-Nab3-Sen1 complex and is degraded by the nuclear exosome. Using a sensitive terminator read-through assay, we identified trans-acting Terminator Override (TOV) genes that operate this terminator. Four genes were identified: the RNA polymerase II phosphatase SSU72, the RNA polymerase II binding protein PCF11, the TRAMP subunit TRF4 and the hnRNP-like, NAB3. The TOV phenotype can be explained by the loss of function of these gene products as described in models in which termination and RNA degradation are coupled to the phosphorylation state of RNA polymerase II's repeat domain. The most interesting mutations were those found in NAB3, which led to the finding that the removal of merely three carboxy-terminal amino acids compromised Nab3's function. This region of previously unknown function is distant from the protein's well-known RNA binding and Nrd1 binding domains. Structural homology modeling suggests this Nab3 'tail' forms an α-helical multimerization domain that helps assemble it onto an RNA substrate.
- Subjects :
- Saccharomyces cerevisiae Proteins
Transcription, Genetic
Termination factor
Molecular Sequence Data
RNA-dependent RNA polymerase
RNA polymerase II
Cell Separation
DNA-Directed DNA Polymerase
Saccharomyces cerevisiae
Biology
03 medical and health sciences
IMP Dehydrogenase
Transcription (biology)
Genetics
Amino Acid Sequence
RNA polymerase II holoenzyme
Alleles
030304 developmental biology
Terminator Regions, Genetic
0303 health sciences
030302 biochemistry & molecular biology
Nuclear Proteins
RNA-Binding Proteins
Flow Cytometry
Protein Structure, Tertiary
Terminator (genetics)
Biochemistry
Mutation
biology.protein
RNA
RNA Polymerase II
Transcription factor II D
Sequence Alignment
Small nuclear RNA
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....1d874130d7b978b4805826b2f3c6d4d9