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Spatiotemporal Patterns of RING1 Expression after Rat Spinal Cord Injury
- Source :
- Neurochemical Research. 42:1191-1201
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Ring finger protein 1 (RING1) is a RING domain characterized protein belonging to the RING finger family. It is an E3 ubiquitin-protein ligase that mediated monoubiquitination of histone H2A and the core component of PRC1 complex, which is the repressive multiprotein complex of Polycomb group (PcG). Previous studies showed the important tumorigenic role of RING1 via promoting cell proliferation and the crucial function in maintaining transcriptional program stability during development. However, its mechanism for spinal cord injury (SCI) is still unknown. In our research, we established an acute SCI model in adult rats and studied the expression and function profiles of RING1. RING1 protein level detected by western blot peaked at day 3 after trauma and then decreased gradually. Immunohistochemistry showed the increase of RING1 expression displayed in the white matter more obviously than in the gray matter. Furthermore, increased co-expression of RING1 and GFAP confirmed activated astrocytes in injured spinal cord via double immunofluorescence staining. Meanwhile, we also found the co-localization of PCNA, a famous marker of proliferative cells, with RING1 and GFAP, which indicated RING1 might play a role in astrocyte proliferation after SCI. In vitro studies, RING1 protein level in C6 cells increased after LPS challenge and RING1 was required for astrocyte proliferation and activation induced by LPS. In summary, we took a new insight into the function of RING1 in the cellular and molecular mechanism underlying the pathophysiology of SCI.
- Subjects :
- Male
0301 basic medicine
Time Factors
Ubiquitin-Protein Ligases
Gene Expression
Biology
Biochemistry
Rats, Sprague-Dawley
03 medical and health sciences
Cellular and Molecular Neuroscience
Western blot
Ring finger
medicine
Animals
PRC1 complex
Spinal cord injury
Spinal Cord Injuries
Polycomb Repressive Complex 1
medicine.diagnostic_test
Cell growth
General Medicine
medicine.disease
Spinal cord
Rats
Cell biology
Proliferating cell nuclear antigen
030104 developmental biology
medicine.anatomical_structure
Spinal Cord
biology.protein
Neuroglia
Astrocyte
Subjects
Details
- ISSN :
- 15736903 and 03643190
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....1d70496d85d2ac9cb4553afe7191f9d5