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The nutrient-sensing repertoires of mouse enterochromaffin cells differ between duodenum and colon
- Source :
- Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society. 29(6)
- Publication Year :
- 2016
-
Abstract
- Background: Enterochromaffin (EC) cells within the gastrointestinal (GI) tract provide almost all body serotonin (5-hydroxytryptamine [5-HT]). Peripheral 5-HT, released from EC cells lining the gut wall, serves diverse physiological roles. These include modulating GI motility, bone formation, hepatic gluconeogenesis, thermogenesis, insulin resistance, and regulation of fat mass. Enterochromaffin cells are nutrient sensors, but which nutrients they are responsive to and how this changes in different parts of the GI tract are poorly understood. Methods: To accurately undertake such an examination, we undertook the first isolation and purification of primary mouse EC cells from both the duodenum and colon in the same animal. This allowed us to compare, in an internally controlled manner, regional differences in the expression of nutrient sensors in EC cells using real-time PCR. Key Results: Both colonic and duodenal EC cells expressed G protein-coupled receptors and facilitative transporters for sugars, free fatty acids, amino acids, and lipid amides. We find differential expression of nutrient receptor and transporters in EC cells obtained from duodenal and colonic EC cells. Duodenal EC cells have higher expression of tryptophan hydroxylase-1, sugar transporters GLUT2, GLUT5, and free fatty acid receptors 1 and 3 (FFAR1 and FFAR3). Colonic EC cells express higher levels of GLUT1, FFAR2, and FFAR4. Conclusions & Inferences: We highlight the diversity of EC cell physiology and identify differences in the regional sensing repertoire of EC cells to an assortment of nutrients. These data indicate that not all EC cells are similar and that differences in their physiological responses are likely dependent on their location within the GI tract. Refereed/Peer-reviewed
- Subjects :
- 0301 basic medicine
Cell physiology
Male
Physiology
Colon
Duodenum
receptors
Motility
Gene Expression
Nutrient sensing
Biology
enterochromaffin
Receptors, G-Protein-Coupled
03 medical and health sciences
nutrients
Free fatty acid receptor 1
Enterochromaffin Cells
Animals
glucose
Receptor
chemistry.chemical_classification
Endocrine and Autonomic Systems
Gastroenterology
Fatty acid
serotonin
030104 developmental biology
Biochemistry
chemistry
Enterochromaffin cell
Mice, Inbred CBA
Serotonin
Subjects
Details
- ISSN :
- 13652982
- Volume :
- 29
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society
- Accession number :
- edsair.doi.dedup.....1d5adc54e9430ac9b81bf457a9c00258