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Evaluation of LR white resin for histology of the undecalcified rat tibia
- Source :
- Biotechnichistochemistry : official publication of the Biological Stain Commission. 75(1)
- Publication Year :
- 2000
-
Abstract
- Histology of plastic embedded undecalcified bone represents a challenging problem to the histotechnologist. We outline here an exploration of LR White resin as a suitable medium for histologic study of undecalcified rat tibia. A procedure was developed for light microscopy of rat tibia embedded in LR White and sectioned by sawing-grinding technics. The specimens were fixed in 10% neutral buffered formalin or alcohol-acetic acid-formol, dehydrated in ethanol, defatted in chloroform followed by resin infiltration and heat-curing of embedded blocks. The procedure of dehydration, defatting, infiltration, and polymerization can be completed within 10 days. Cold curing with accelerator provided by the manufacturer did not yield superior results compared to blocks cured with heat. Thick sections were obtained using a diamond wire saw, attached to plexiform slides, then ground and polished. Surface staining with Von Kossa silver reagent or toluidine blue revealed satisfactory morphological preservation of the mineralized bone sections. Artifacts like small bubbles appeared occasionally and could not be avoided despite prolonged infiltration or cold curing of blocks. Our method is relatively simple for base-line histologic study of rat tibia. The method offers advantages such as easy adaptability, reliable stainability, contrast, and resolution of bone architecture and marrow cells. Two other embedding media, Micro-Bed resin and Unicryl, were also tested, but produced inferior results.
- Subjects :
- Histology
Materials science
Plastic Embedding
Staining and Labeling
Tibia
Acrylic Resins
General Medicine
Anatomy
Defatting
Staining
Rats
Embedding Medium
Medical Laboratory Technology
Evaluation Studies as Topic
visual_art
visual_art.visual_art_medium
Animals
Von Kossa stain
Acrylic resin
Curing (chemistry)
Biomedical engineering
Subjects
Details
- ISSN :
- 10520295
- Volume :
- 75
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biotechnichistochemistry : official publication of the Biological Stain Commission
- Accession number :
- edsair.doi.dedup.....1d5422039665f9d77ab912e3005a8f79