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A new multidrug‐resistant enterotoxigenic Escherichia coli pulsed‐field gel electrophoresis cluster associated with enrofloxacin non‐susceptibility in diseased pigs
- Source :
- Journal of Applied Microbiology
- Publication Year :
- 2020
- Publisher :
- John Wiley and Sons Inc., 2020.
-
Abstract
- Aims To describe the temporal trends in Escherichia coli pathotypes and antimicrobial resistance detected in isolates from diseased‐pig cases submitted to the EcL from 2008 to 2016, in Quebec, Canada, and to investigate the presence of spatiotemporal and phylogenetic clusters. Methods and Results Detection of 12 genes coding for virulence factors in pathogenic E. coli in pigs by PCR and antimicrobial resistance standard disc diffusion assay were performed. Demographic and clinical data were entered in the Animal Pathogenic and Zoonotic E. coli (APZEC) database. ETEC:F4 was the most prevalent pathovirotype among the 3773 cases submitted. The LT:STb:F4 virotype was predominant until 2014, then was overtaken by the LT:STb:STa:F4 virotype. More than 90% of the ETEC:F4 isolates were multidrug resistant. A spatiotemporal cluster of LT:STb:STa:F4 isolates non‐susceptible to enrofloxacin was detected between 4/2015 and 9/2016. Pulsed‐field gel electrophoresis analysis of 137 ETEC:F4 isolates revealed the presence of a cluster composed mainly of LT:STb:STa:F4 isolates non‐susceptible to enrofloxacin. Conclusions The APZEC database was useful to highlight temporal trends in E. coli pathotypes. A high‐risk ETEC:F4 clone might disseminate in the pig population in Quebec since 2015. Significance and Impact of the Study Surveillance is crucial to identify new clones and develop control strategies.
- Subjects :
- Canada
Databases, Factual
Swine
Virulence Factors
Population
Animal Microbiology/Animal Health Microbiology
Virulence
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Antibiotic resistance
Enterotoxigenic Escherichia coli
Drug Resistance, Multiple, Bacterial
Enrofloxacin
medicine
Pulsed-field gel electrophoresis
Escherichia coli
Animals
antimicrobial resistance
education
fluoroquinolones non‐susceptibility
Escherichia coli Infections
Phylogeny
030304 developmental biology
Swine Diseases
0303 health sciences
education.field_of_study
030306 microbiology
clonal complex
ETEC
pigs
General Medicine
Original Articles
Electrophoresis, Gel, Pulsed-Field
Multiple drug resistance
Original Article
Biotechnology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 13652672 and 13645072
- Volume :
- 130
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Microbiology
- Accession number :
- edsair.doi.dedup.....1d4861ce465ae8edc72e191c5424ce25