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Molecular characterisation of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone in a Mexican hospital (1999–2003)

Authors :
S. Esparza-Ahumada
H. de Lencastre
Marta Aires-de-Sousa
Maria Elena Velazquez-Meza
Carnalla-Barajas N
Eduardo Rodríguez-Noriega
Rayo Morfin-Otero
Gabriela Echániz-Aviles
Source :
Clinical Microbiology and Infection. 12:22-28
Publication Year :
2006
Publisher :
Elsevier BV, 2006.

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) isolates ( n = 216), collected between January 1999 and May 2003 in a tertiary-care university hospital in Guadalajara, Mexico, were characterised by antibiotype, pulsed-field gel electrophoresis (PFGE) of Sma I macrorestriction fragments, and hybridisation of Cla I digests with mecA - and Tn 554 -specific DNA probes. Representatives of the single clonal type found were analysed by spa typing, multilocus sequence typing and staphylococcal chromosomal cassette mec (SCC mec ) typing, and were tested for the presence of 22 virulence determinants and agr type. A single PFGE pattern was identified, with minor variations over time, with spa type 2, sequence type 5, SCC mec type II, agr type 2 and the presence of the enterotoxin genes seg and sei , the γ-haemolysin variant gene hlg-v and the leukocidin lukE–lukD genes. In addition, the isolates showed antimicrobial resistance to β-lactams, macrolides, chloramphenicol and imipenem, and susceptibility to gentamicin, rifampicin, trimethoprim–sulphamethoxazole and vancomycin. Following its appearance in 1997, this clone spread within the hospital, and is now present in most of the hospital units and wards.

Details

ISSN :
1198743X
Volume :
12
Database :
OpenAIRE
Journal :
Clinical Microbiology and Infection
Accession number :
edsair.doi.dedup.....1d3729da6cb7c0e0afea43c5c3c6f062
Full Text :
https://doi.org/10.1111/j.1469-0691.2005.01283.x