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Molecular characterisation of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone in a Mexican hospital (1999–2003)
- Source :
- Clinical Microbiology and Infection. 12:22-28
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Methicillin-resistant Staphylococcus aureus (MRSA) isolates ( n = 216), collected between January 1999 and May 2003 in a tertiary-care university hospital in Guadalajara, Mexico, were characterised by antibiotype, pulsed-field gel electrophoresis (PFGE) of Sma I macrorestriction fragments, and hybridisation of Cla I digests with mecA - and Tn 554 -specific DNA probes. Representatives of the single clonal type found were analysed by spa typing, multilocus sequence typing and staphylococcal chromosomal cassette mec (SCC mec ) typing, and were tested for the presence of 22 virulence determinants and agr type. A single PFGE pattern was identified, with minor variations over time, with spa type 2, sequence type 5, SCC mec type II, agr type 2 and the presence of the enterotoxin genes seg and sei , the γ-haemolysin variant gene hlg-v and the leukocidin lukE–lukD genes. In addition, the isolates showed antimicrobial resistance to β-lactams, macrolides, chloramphenicol and imipenem, and susceptibility to gentamicin, rifampicin, trimethoprim–sulphamethoxazole and vancomycin. Following its appearance in 1997, this clone spread within the hospital, and is now present in most of the hospital units and wards.
- Subjects :
- Adult
DNA, Bacterial
Microbiology (medical)
Staphylococcus aureus
Imipenem
Meticillin
Adolescent
Genotype
hospital clones
Epidemiology
Microbial Sensitivity Tests
MRSA
Biology
medicine.disease_cause
Microbiology
Hospitals, University
03 medical and health sciences
methicillin resistance
0302 clinical medicine
Pulsed-field gel electrophoresis
medicine
Humans
030212 general & internal medicine
Typing
Child
Mexico
Aged
Antibacterial agent
Aged, 80 and over
0303 health sciences
Virulence
030306 microbiology
Infant
General Medicine
Middle Aged
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis, Gel, Pulsed-Field
3. Good health
Phenotype
Infectious Diseases
Child, Preschool
Multilocus sequence typing
medicine.drug
Subjects
Details
- ISSN :
- 1198743X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Clinical Microbiology and Infection
- Accession number :
- edsair.doi.dedup.....1d3729da6cb7c0e0afea43c5c3c6f062
- Full Text :
- https://doi.org/10.1111/j.1469-0691.2005.01283.x