Back to Search
Start Over
Dehydroascorbic acid recycled by thiols efficiently scavenges non-thermal plasma-induced hydroxyl radicals
- Source :
- Archives of Biochemistry and Biophysics. 669:87-95
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Recent development in electronics has enabled the use of non-thermal plasma (NTP) to strictly direct oxidative stress in a defined location at near-physiological temperature. In preclinical studies or human clinical trials, NTP promotes blood coagulation, wound healing with disinfection, and selective killing of cancer cells. Although these biological effects of NTP have been widely explored, the stoichiometric quantitation of free radicals in liquid phase has not been performed in the presence of biocompatible reducing agents, which may modify the final biological effects of NTP. Here we quantitated hydroxyl radicals, a major reactive oxygen species generated after NTP exposure, by electron paramagnetic resonance (EPR) spectroscopy using two distinct spin-trapping probes, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO), in the presence of thiols or antioxidants. l -Ascorbic acid (AsA) at 25–50 μM concentrations (physiological concentration in the serum) significantly scavenged these hydroxyl radicals, whereas dithiothreitol (DTT), reduced glutathione (GSH), and N-acetyl-cysteine (NAC) as thiols were required in millimolar concentrations to perform scavenging activities. l -Dehydroascorbic acid (DHA), an oxidized form of AsA, necessitated the presence of 25–50 μM DTT or sub-millimolar concentrations of GSH and NAC for the scavenging of hydroxyl radicals and failed to scavenge hydroxyl radicals by itself. These results suggest that the redox cycling of AsA/DHA via thiols and cellular AsA metabolism are important processes to be considered while applying NTP to cells and tissues. Further studies are warranted to elucidate the interaction between other reactive species generated by NTP and biomolecules to promote biological and medical applications of NTP.
- Subjects :
- 0301 basic medicine
Plasma Gases
Reducing agent
viruses
Radical
Biophysics
Ascorbic Acid
medicine.disease_cause
Biochemistry
Dithiothreitol
Cyclic N-Oxides
03 medical and health sciences
chemistry.chemical_compound
medicine
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
030102 biochemistry & molecular biology
Hydroxyl Radical
Electron Spin Resonance Spectroscopy
Free Radical Scavengers
Glutathione
Ascorbic acid
Dehydroascorbic Acid
Combinatorial chemistry
Acetylcysteine
030104 developmental biology
chemistry
Spin Labels
Dehydroascorbic acid
Oxidative stress
Subjects
Details
- ISSN :
- 00039861
- Volume :
- 669
- Database :
- OpenAIRE
- Journal :
- Archives of Biochemistry and Biophysics
- Accession number :
- edsair.doi.dedup.....1d2f807bdfbaa2ec8fa624c7916b430f