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Connexin43 repression following epithelium-to-mesenchyme transition in embryonal carcinoma cells requires Snail1 transcription factor
- Source :
- Differentiation; research in biological diversity, 75(3), 208-218. Elsevier BV
- Publication Year :
- 2007
-
Abstract
- Embryonic stem (ES) cells and embryonal carcinoma (EC) cells express high amounts of functional connexin43 (Cx43). During mesoderm formation and subsequent cardiac differentiation, Cx43 is initially down-regulated but is up-regulated again as the emerging cardiomyocytes mature. In this study, we investigated the regulation of Cx43 expression during early phases of differentiation in F9 and P19 EC cells. We found a striking inverse correlation between the expression of Cx43 and that of the transcriptional repressor Snail1. No clear relationship was found with Smad-interacting-protein1 (SIP1), another transcription factor inducing epithelium-to-mesenchyme transition (EMT). Promoter-reporter assays indicated Cx43 repression at the promoter level by ectopically expressed Snail1. To establish whether the Cx43 down-regulation depends on endogenous Snail1, MES-1 cells, differentiated derivatives of P19 EC, were stably transfected by an siRNA construct silencing Snail1 expression. This resulted in a mesenchyme-to-epithelium transition, which was accompanied by increased levels of Cx43 mRNA and protein and enhanced metabolic and electrical coupling. We conclude that Snail1-mediated EMT results in a Cx43 repression.
- Subjects :
- Cancer Research
Mesenchyme
Down-Regulation
Fluorescent Antibody Technique
Biology
Transfection
Epithelium
Mesoderm
Embryonal carcinoma
Mice
Carcinoma, Embryonal
Cell Line, Tumor
medicine
Animals
Gene silencing
Gene Silencing
RNA, Messenger
RNA, Small Interfering
Promoter Regions, Genetic
Molecular Biology
Psychological repression
Transcription factor
Embryonic Stem Cells
Cell Biology
medicine.disease
Embryonic stem cell
Molecular biology
medicine.anatomical_structure
Connexin 43
Mesoderm formation
cardiovascular system
Snail Family Transcription Factors
sense organs
biological phenomena, cell phenomena, and immunity
Transcription Factors
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 03014681
- Volume :
- 75
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Differentiation; research in biological diversity
- Accession number :
- edsair.doi.dedup.....1d27ac89e30b9bfd02f68ad109f7990d