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Disruption of Trim9 function abrogates macrophage motility in vivo
- Source :
- Journal of Leukocyte Biology. 102:1371-1380
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- The vertebrate immune response comprises multiple molecular and cellular components that interface to provide defense against pathogens. Because of the dynamic complexity of the immune system and its interdependent innate and adaptive functionality, an understanding of the whole-organism response to pathogen exposure remains unresolved. Zebrafish larvae provide a unique model for overcoming this obstacle, because larvae are protected against pathogens while lacking a functional adaptive immune system during the first few weeks of life. Zebrafish larvae were exposed to immune agonists for various lengths of time, and a microarray transcriptome analysis was executed. This strategy identified known immune response genes, as well as genes with unknown immune function, including the E3 ubiquitin ligase tripartite motif-9 (Trim9). Although trim9 expression was originally described as “brain specific,” its expression has been reported in stimulated human Mϕs. In this study, we found elevated levels of trim9 transcripts in vivo in zebrafish Mϕs after immune stimulation. Trim9 has been implicated in axonal migration, and we therefore investigated the impact of Trim9 disruption on Mϕ motility and found that Mϕ chemotaxis and cellular architecture are subsequently impaired in vivo. These results demonstrate that Trim9 mediates cellular movement and migration in Mϕs as well as neurons.
- Subjects :
- 0301 basic medicine
Ubiquitin-Protein Ligases
Immunology
Motility
Nerve Tissue Proteins
Cell Development, Differentiation, & Trafficking
Tripartite Motif Proteins
Transcriptome
03 medical and health sciences
Immune system
Cell Movement
Animals
Humans
Immunology and Allergy
Macrophage
RNA, Messenger
Cell Shape
Zebrafish
biology
Chemotaxis
Macrophages
U937 Cells
Cell Biology
Zebrafish Proteins
biology.organism_classification
Acquired immune system
Ubiquitin ligase
Cell biology
030104 developmental biology
biology.protein
Subjects
Details
- ISSN :
- 19383673 and 07415400
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Journal of Leukocyte Biology
- Accession number :
- edsair.doi.dedup.....1d23a8aa66f00f3e6c17580613047c83