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The Mitochondrial Targets of Neuroprotective Drug Vinpocetine on Primary Neuron Cultures, Brain Capillary Endothelial Cells, Synaptosomes, and Brain Mitochondria
- Source :
- Neurochemical Research
- Publication Year :
- 2019
- Publisher :
- Springer US, 2019.
-
Abstract
- Vinpocetine is considered as neuroprotectant drug and used for treatment of brain ischemia and cognitive deficiencies for decades. A number of enzymes, channels and receptors can bind vinpocetine, however the mechanisms of many effects’ are still not clear. The present study investigated the effects of vinpocetine from the mitochondrial bioenergetic aspects. In primary brain capillary endothelial cells the purinergic receptor-stimulated mitochondrial Ca2+ uptake and efflux were studied. Vinpocetine exerted a partial inhibition on the mitochondrial calcium efflux. In rodent brain synaptosomes vinpocetine (30 μM) inhibited respiration in uncoupler stimulated synaptosomes and decreased H2O2 release from the nerve terminals in resting and in complex I inhibited conditions, respectively. In isolated rat brain mitochondria using either complex I or complex II substrates leak respiration was stimulated, but ADP-induced respiration was inhibited by vinpocetine. The stimulation of oxidation was associated with a small extent of membrane depolarization. Mitochondrial H2O2 production was inhibited by vinpocetine under all conditions investigated. The most pronounced effects were detected with the complex II substrate succinate. Vinpocetine also mitigated both Ca2+-induced mitochondrial Ca2+-release and Ca2+-induced mitochondrial swelling. It lowered the rate of mitochondrial ATP synthesis, while increasing ATPase activity. These results indicate more than a single mitochondrial target of this vinca alkaloid. The relevance of the affected mitochondrial mechanisms in the anti ischemic effect of vinpocetine is discussed.
- Subjects :
- 0301 basic medicine
Vinpocetine
medicine.drug_class
Oxygen consumption
Mitochondrion
Pharmacology
Biochemistry
Neuroprotection
Uncoupling
Vinca alkaloid
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
medicine
Animals
Rats, Wistar
Vinca Alkaloids
ATP synthesis
Membrane Potential, Mitochondrial
Neurons
Original Paper
Chemistry
Purinergic receptor
Brain
Endothelial Cells
Depolarization
General Medicine
Hydrogen Peroxide
Mitochondria
030104 developmental biology
medicine.anatomical_structure
Neuroprotective Agents
Calcium
Neuron
Reactive oxygen species
Calcium-induced calcium release
030217 neurology & neurosurgery
medicine.drug
Calcium induced calcium release
Synaptosomes
Subjects
Details
- Language :
- English
- ISSN :
- 15736903 and 03643190
- Volume :
- 44
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....1d22d0a4849ae2e29e9bea120b454ba9