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Differences in infectivity between endosymbiotic Chlorella variabilis cultivated outside host Paramecium bursaria for 50 years and those immediately isolated from host cells after one year of reendosymbiosis
- Source :
- Biology Open, Biology Open, Vol 5, Iss 1, Pp 55-61 (2016)
- Publication Year :
- 2015
- Publisher :
- The Company of Biologists, 2015.
-
Abstract
- Chlorella variabilis strain NC64A is an intracellular photobiont of the ciliate Paramecium bursaria. NC64A was isolated from P. bursaria nearly 50 years ago and was thereafter cultivated outside the host. This study was undertaken to detect changes in its infectivity to P. bursaria and its auxotrophy for growth outside the host induced during long-term cultivation. NC64A can grow in Modified Bold's Basal Medium but not in C medium, whereas another symbiotic Chlorella variabilis strain, 1N, that was recently isolated from the host grew in C medium but not in Modified Bold's Basal Medium. With regards infectivity, NC64A in the logarithmic phase of growth showed low infectivity to alga-removed P. bursaria cells, whereas those in the early stationary phase showed high infectivity of about 30%. Those in the decay phase of growth showed no infectivity. Results show that NC64A has infectivity, but the infection rate depends on their culture age in the growth curve. Furthermore, NC64A that had been re-infected to P. bursaria for more than one year and isolated from the host showed a nearly 100% infection rate, which indicates that NC64A can recover its infectivity by re-infection to P. bursaria.<br />Summary: This study was undertaken to detect changes in infectivity induced during long-term cultivation of Chlorella variabilis to alga-free Paramecium bursaria, and its auxotrophy for growth outside the host.
- Subjects :
- 0301 basic medicine
QH301-705.5
Science
Auxotrophy
Long-term cultivation
Bursaria
Chlorella variabilis
General Biochemistry, Genetics and Molecular Biology
Microbiology
03 medical and health sciences
Botany
Biology (General)
Infectivity
Ciliate
Endosymbiosis
biology
Strain (chemistry)
Host (biology)
biology.organism_classification
Paramecium bursaria
030104 developmental biology
General Agricultural and Biological Sciences
Research Article
Subjects
Details
- ISSN :
- 20466390
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Biology Open
- Accession number :
- edsair.doi.dedup.....1d2296d2a70b2e692fdcc6d91489b922