Back to Search
Start Over
Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without
- Source :
- iScience, 24(3):102170, iScience, iScience, Vol 24, Iss 3, Pp 102170-(2021)
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- Cell entry of the pandemic virus SARS-CoV-2 is mediated by its spike protein S. As main antigenic determinant, S protein is in focus of various therapeutic strategies. Besides particle-cell fusion, S mediates fusion between infected and uninfected cells resulting in syncytia formation. Here we present sensitive assay systems with a high dynamic range and high signal-to-noise ratios covering not only particle-cell and cell-cell fusion, but also fusion-from-without (FFWO). In FFWO, S-containing viral particles induce syncytia independently of de novo synthesis of S. Neutralizing antibodies as well as sera from convalescent patients inhibited particle-cell fusion with high efficiency. Cell-cell fusion, in contrast, was only moderately inhibited despite requiring levels of S protein below the detection limit of flow cytometry and Western blot. The data indicate that syncytia formation as pathological consequence during Covid-19 can proceed at low levels of S protein and may not be effectively prevented by antibodies.<br />Graphical Abstract
- Subjects :
- 0301 basic medicine
viruses
Membrane Architecture
02 engineering and technology
Article
Virus
Epitope
Flow cytometry
03 medical and health sciences
Western blot
medicine
lcsh:Science
Biochemical Assay
Fusion
Syncytium
Multidisciplinary
Cell fusion
medicine.diagnostic_test
biology
Chemistry
Assay
COVID-19
Lipid bilayer fusion
Cell Biology
021001 nanoscience & nanotechnology
Virology
Molecular biology
030104 developmental biology
biology.protein
lcsh:Q
Antibody
0210 nano-technology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- iScience, 24(3):102170, iScience, iScience, Vol 24, Iss 3, Pp 102170-(2021)
- Accession number :
- edsair.doi.dedup.....1d1008314db7b04665d7a8863a684474