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Intestinal Oxidative State Can Alter Nutrient and Drug Bioavailability
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2, Iss 5, Pp 322-327 (2009), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2009
- Publisher :
- Hindawi Limited, 2009.
-
Abstract
- Organic cations (OCs) are substances of endogenous (e.g., dopamine, choline) or exogenous (e.g., drugs like cimetidine) origin that are positively charged at physiological ph. since many of these compounds can not pass the cell membrane freely, their transport in or out of cells must be mediated by specific transport systems. Transport by organic cation transporters (OCTs) can be regulated rapidly by altering their trafficking and/or affinities in response to stimuli. However, for example, a specific disease could lead to modifications in the expression of OCTs. Chronic exposure to oxidative stress has been suggested to alter regulation and functional activity of proteins through several pathways. According to results from a previous work, oxidation-reduction pathways were thought to be involved in intestinal organic cation uptake modulation. The present work was performed in order to evaluate the influence of oxidative stressors, especially glutathione, on the intestinal organic cation absorption. For this purpose, the effect of compounds with different redox potential (glutathione, an endogenous antioxidant, and procyanidins, diet antioxidants) was assessed on MPP+(1-methyl-4-phenylpyridinium iodide) uptake in an enterocyte cell line (Caco-2). Caco-2 cells were subcultured with two different media conditions (physiological: 5 mM glucose, referred as control cells; and high-glucose: 25 mM glucose, referred as HG cells). In HG cells, the uptake was significantly lower than in control cells. Redox changing interventions affected Mpp+uptake, both in control and in high-glucose Caco-2 cells. Cellular glutathione levels could have an important impact on membrane transporter activity. The results indicate that modifications in the cellular oxidative state modulate MPP+uptake by Caco-2 cells. Such modifications may reflect in changes of nutrient and drug bioavailability.
- Subjects :
- 1-Methyl-4-phenylpyridinium
Aging
Antioxidant
oxidation-reduction
Organic Cation Transport Proteins
medicine.medical_treatment
Biological Availability
Endogeny
Oxidative phosphorylation
medicine.disease_cause
Biochemistry
Cell membrane
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cations
medicine
Humans
Choline
Proanthocyanidins
lcsh:QH573-671
030304 developmental biology
0303 health sciences
Organic cation transport proteins
biology
lcsh:Cytology
030206 dentistry
Cell Biology
General Medicine
Glutathione
MPP+
Oxidative Stress
Enterocytes
Glucose
medicine.anatomical_structure
Pharmaceutical Preparations
chemistry
biology.protein
OCTs (organic cation transporters)
Caco-2 Cells
Oxidative stress
Research Paper
Subjects
Details
- ISSN :
- 19420994 and 19420900
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....1d0bd4106f99687cf44f61b6568bc3fd