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Mitochondrial RNase P RNAs in ascomycete fungi: Lineage-specific variations in RNA secondary structure

Authors :
Elias Seif
Nancy C. Martin
Lise Forget
B. Franz Lang
Source :
RNA. 9:1073-1083
Publication Year :
2003
Publisher :
Cold Spring Harbor Laboratory, 2003.

Abstract

The RNA subunit of mitochondrial RNase P (mtP-RNA) is encoded by a mitochondrial gene (rnpB) in several ascomycete fungi and in the protists Reclinomonas americana and Nephroselmis olivacea. By searching for universally conserved structural elements, we have identified previously unknown rnpB genes in the mitochondrial DNAs (mtDNAs) of two fission yeasts, Schizosaccharomyces pombe and Schizosaccharomyces octosporus; in the budding yeast Pichia canadensis; and in the archiascomycete Taphrina deformans. The expression of mtP-RNAs of the predicted size was experimentally confirmed in the two fission yeasts, and their precise 5′ and 3′ ends were determined by sequencing of cDNAs generated from circularized mtP-RNAs. Comparative RNA secondary structure modeling shows that in contrast to mtP-RNAs of the two protists R. americana and N. olivacea, those of ascomycete fungi all have highly reduced secondary structures. In certain budding yeasts, such as Saccharomycopsis fibuligera, we find only the two most conserved pairings, P1 and P4. A P18 pairing is conserved in Saccharomyces cerevisiae and its close relatives, whereas nearly half of the minimum bacterial consensus structure is retained in the RNAs of fission yeasts, Aspergillus nidulans and Taphrina deformans. The evolutionary implications of the reduction of mtP-RNA structures in ascomycetes will be discussed.

Details

ISSN :
14699001 and 13558382
Volume :
9
Database :
OpenAIRE
Journal :
RNA
Accession number :
edsair.doi.dedup.....1d08254a55d2cc77ee5e5c825d562185
Full Text :
https://doi.org/10.1261/rna.5880403